NWIPB OpenIR
A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging
Guo, Lan1; Yang, Daoshan1; Xia, Lian1; Qu, Fei1; Dou, Yanan1; Qu, Fengli1; Kong, Rongmei1; You, Jinmao1,2
2017-12-21
Source PublicationNEW JOURNAL OF CHEMISTRY
AbstractIn this study, a coumarin-based probe (probe 1) bearing a maleimide group was used to rapidly and selectively detect thiols. After inducing thiols, this probe presented a significant fluorescence enhancement (>22-fold), whereas other amino acids that had no sulfhydryl group just generated slight changes. The detection limits were determined to be as low as 7.41 nM for Cys, 6.06 nM for GSH, and 7.73 nM for Hcy based on S/N = 3. Furthermore, this probe possessed excellent water solubility and successfully detected thiols in PBS (pH 7.4, 99.5%). More importantly, the practical application of probe 1 for the selective detection of thiols was successfully demonstrated in living T24 cells.
PublisherROYAL SOC CHEMISTRY
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/23767
Collection中国科学院西北高原生物研究所
Corresponding AuthorXia, Lian
Affiliation1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China
2.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining, Qinghai, Peoples R China
Recommended Citation
GB/T 7714
Guo, Lan,Yang, Daoshan,Xia, Lian,et al. A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging[J]. NEW JOURNAL OF CHEMISTRY,2017.
APA Guo, Lan.,Yang, Daoshan.,Xia, Lian.,Qu, Fei.,Dou, Yanan.,...&You, Jinmao.(2017).A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging.NEW JOURNAL OF CHEMISTRY.
MLA Guo, Lan,et al."A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging".NEW JOURNAL OF CHEMISTRY (2017).
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