A homogalacturonan from Hippophae rhamnoides L. Berries enhance immunomodulatory activity through TLR4/MyD88 pathway mediated activation of macrophages

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	A homogalacturonan from Hippophae rhamnoides L. Berries enhance immunomodulatory activity through TLR4/MyD88 pathway mediated activation of macrophages
	Wang, Hailiang ; Bi, Hongtao ; Gao, Tingting ; Zhao, Bin ; Ni, Weihua ; Liu, Jun
	2018-02-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
摘要	Our previous study isolated a natural high-methoxyl homogalacturonan (HRWP-A) from Hippophae rhamnoides and showed antitumor activity in vivo. In this study, the immunomodulatory activity and mechanisms of action of HRWP-A were further investigated. Results showed that HRWP-A could recover the body condition and activated macrophage in Cyclophosphamide (CTX)-induced immunosuppressed mice. Further, we investigated the possible mechanism underlying the effects of HRWP-A on mouse peritoneal macrophages. qPCR and western blot revealed that HRWP-A upregulated the expression of TLR4 mRNA in vitro. This process was accompanied by a clear increase in MyD88 expression and p-l kappa B-alpha, but these effects were largely abrogated by pretreatment with anti-TLR4 antibodies. The effects of HRWP-A on macrophage NO, IL-1 beta and 1L-6 production were also inhibited by anti-TLR4 antibodies and were greatly influenced by the NF-kappa B inhibitor PDTC. Moreover, HRWP-A failed to induce the production of NO, IL-1 beta and IL-6 in peritoneal macrophages prepared from C3H/HeJ mice, which have a point mutation in the Tlr4 gene, suggesting the involvement of the TLR4 molecule in HRWP-A-mediated macrophage activation. These results may have important implications for our understanding of the structure-activity relationship of immunopotentiating polysaccharides from medicinal herbs. (C) 2017 Elsevier B.V. All rights reserved.
出版者	ELSEVIER SCIENCE BV
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/23793
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Hailiang,Bi, Hongtao,Gao, Tingting,et al. A homogalacturonan from Hippophae rhamnoides L. Berries enhance immunomodulatory activity through TLR4/MyD88 pathway mediated activation of macrophages[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018.
APA	Wang, Hailiang,Bi, Hongtao,Gao, Tingting,Zhao, Bin,Ni, Weihua,&Liu, Jun.(2018).A homogalacturonan from Hippophae rhamnoides L. Berries enhance immunomodulatory activity through TLR4/MyD88 pathway mediated activation of macrophages.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.
MLA	Wang, Hailiang,et al."A homogalacturonan from Hippophae rhamnoides L. Berries enhance immunomodulatory activity through TLR4/MyD88 pathway mediated activation of macrophages".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2018).