A potato NOA gene increased salinity tolerance in Arabidopsis thaliana

NWIPB OpenIR

	A potato NOA gene increased salinity tolerance in Arabidopsis thaliana
	Zhang, B. ; Wang, H. Q. ; Liu, B. L. ; Liu, J. ; Wang, X. ; Liu, Q. ; Zhang, H. G.
	2010-09-06
发表期刊	AFRICAN JOURNAL OF BIOTECHNOLOGY ; Zhang, B; Wang, HQ; Liu, BL; Liu, J; Wang, X; Liu, Q; Zhang, HG.A potato NOA gene increased salinity tolerance in Arabidopsis thaliana,AFRICAN JOURNAL OF BIOTECHNOLOGY,2010,9(36):5869-5878
摘要	The purpose of this study is to produce recombinant StNOA1 in transgenic plants and to test its potential role in plant salt stress responses. The newly cloned StNOA1 gene from Solanum tuberosum L. was inserted into AtnOA1 mutant plant genome by Agrobaterium-mediated floral dip method. Transgene integration was verified by polymerase chain reaction (PCR) in 4 different lines of transgenic Atnoa1. Expression of StNOA1 gene was further analyzed by reverse trancription (RT)-PCR. Physiological analyses indicated that the transgenic line TL9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (MDA) compared to its receptor, Atnoa1 mutant, under salt stress condition. Root elongation and survival rate in TL9 were significantly higher than those in Atnoa1 seedlings under salt stress. Present study proved that StNOA1 participated in Arabidopsis thaliana salt stress responses and increased its salinity tolerance.; The purpose of this study is to produce recombinant StNOA1 in transgenic plants and to test its potential role in plant salt stress responses. The newly cloned StNOA1 gene from Solanum tuberosum L. was inserted into AtnOA1 mutant plant genome by Agrobaterium-mediated floral dip method. Transgene integration was verified by polymerase chain reaction (PCR) in 4 different lines of transgenic Atnoa1. Expression of StNOA1 gene was further analyzed by reverse trancription (RT)-PCR. Physiological analyses indicated that the transgenic line TL9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (MDA) compared to its receptor, Atnoa1 mutant, under salt stress condition. Root elongation and survival rate in TL9 were significantly higher than those in Atnoa1 seedlings under salt stress. Present study proved that StNOA1 participated in Arabidopsis thaliana salt stress responses and increased its salinity tolerance.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/25599
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, B.,Wang, H. Q.,Liu, B. L.,et al. A potato NOA gene increased salinity tolerance in Arabidopsis thaliana[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY, Zhang, B; Wang, HQ; Liu, BL; Liu, J; Wang, X; Liu, Q; Zhang, HG.A potato NOA gene increased salinity tolerance in Arabidopsis thaliana,AFRICAN JOURNAL OF BIOTECHNOLOGY,2010,9(36):5869-5878,2010.
APA	Zhang, B..,Wang, H. Q..,Liu, B. L..,Liu, J..,Wang, X..,...&Zhang, H. G..(2010).A potato NOA gene increased salinity tolerance in Arabidopsis thaliana.AFRICAN JOURNAL OF BIOTECHNOLOGY.
MLA	Zhang, B.,et al."A potato NOA gene increased salinity tolerance in Arabidopsis thaliana".AFRICAN JOURNAL OF BIOTECHNOLOGY (2010).