Isolation and characterization of microsatellite loci in Ostryopsis davidiana (Betulaceae)

NWIPB OpenIR

	Isolation and characterization of microsatellite loci in Ostryopsis davidiana (Betulaceae)
	Qiu, Qiang ; Tian, Bin ; Wen, Hong-Yan ; Wu, Gui-Li ; Wang, Yu-Jin
	2009-06-01
发表期刊	CONSERVATION GENETICS ; Qiu, Q; Tian, B; Wen, HY; Wu, GL; Wang, YJ.Isolation and characterization of microsatellite loci in Ostryopsis davidiana (Betulaceae),CONSERVATION GENETICS,2009,10(3):751-753
摘要	Ostryopsis (Betulaceae) is a samll genus endemic to China with only two species. Both of them play an important role in restoring the local ecosystems. The distribution of genetic diversity between and within populations in each species are important to further utilize the wild genetic resources and explore the interspecific divergence. In this study, we developed 10 microsatellite loci from O. davidiana by the combining biotin capture method for the first time. A total of 27 microsatellite sequences were recovered through screening the library and 10 of them are polymorphic. The number of alleles per locus in 18 sampled individuals ranged from 3 to 6, expected heterozygosity and observed heterozygosity ranged from 0.2958 to 0.4767 and from 0.1591 to 0.2997, respectively. In addition, all markers have been crossly checked in the other congeneric species. These microsatellite markers would together provide a useful tool for investigating the genetic diversity and structure of both species and speciation mechanism between them.; Ostryopsis (Betulaceae) is a samll genus endemic to China with only two species. Both of them play an important role in restoring the local ecosystems. The distribution of genetic diversity between and within populations in each species are important to further utilize the wild genetic resources and explore the interspecific divergence. In this study, we developed 10 microsatellite loci from O. davidiana by the combining biotin capture method for the first time. A total of 27 microsatellite sequences were recovered through screening the library and 10 of them are polymorphic. The number of alleles per locus in 18 sampled individuals ranged from 3 to 6, expected heterozygosity and observed heterozygosity ranged from 0.2958 to 0.4767 and from 0.1591 to 0.2997, respectively. In addition, all markers have been crossly checked in the other congeneric species. These microsatellite markers would together provide a useful tool for investigating the genetic diversity and structure of both species and speciation mechanism between them.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/25707
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Qiu, Qiang,Tian, Bin,Wen, Hong-Yan,et al. Isolation and characterization of microsatellite loci in Ostryopsis davidiana (Betulaceae)[J]. CONSERVATION GENETICS, Qiu, Q; Tian, B; Wen, HY; Wu, GL; Wang, YJ.Isolation and characterization of microsatellite loci in Ostryopsis davidiana (Betulaceae),CONSERVATION GENETICS,2009,10(3):751-753,2009.
APA	Qiu, Qiang,Tian, Bin,Wen, Hong-Yan,Wu, Gui-Li,&Wang, Yu-Jin.(2009).Isolation and characterization of microsatellite loci in Ostryopsis davidiana (Betulaceae).CONSERVATION GENETICS.
MLA	Qiu, Qiang,et al."Isolation and characterization of microsatellite loci in Ostryopsis davidiana (Betulaceae)".CONSERVATION GENETICS (2009).