Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD

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	Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD
	Li, Guoliang ; Song, Cuihua ; You, Jinmao ; Zhang, Xiaolong ; Chen, Guang
	2012-04-01
发表期刊	JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY ; Guoliang Li;Cuihua Song;Jinmao You;Xiaolong Zhang;Guang Chen.Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD,Journal of the American Oil Chemists' Society,2011,28():
摘要	Free fatty acids (FFA) are of the basic and indispensable components in medicinal plants. Many researches indicate the efficacy of medicinal plant require the presence of FFA. In the present study, ultrasonic-assisted extraction of FFA from swertia species was optimized by response surface methodology (RSM), ensuring the highest FFA recoveries. A novel pre-column fluorescence labelling method using 2-(5-Benzoacridine) ethyl-p-toluenesulfonate (BAETS) as labeling reagent has been developed for highly sensitive and selective analysis of FFA by HPLC with fluorescent detection (FLD) and online mass spectrometry identification. RSM was also employed to optimize the fluorescence labelling of FFA. HPLC separation of 17 FFA derivatives was carried out on a reversed-phase Hypersil BDS C8 column (4.6 ×200 mm, 5 μm, Agilent Co.) with a gradient elution. The detection limits and quantifications were as low as 0.60 and 1.22 ng mL-1, respectively. FFA analysis from 9 swertia species was performed by the newly developed method, and the FFA composition in these swertia samples was first reported.; Free fatty acids (FFA) are basic and indispensable components of medicinal plants. Many researches indicate that the efficacy of medicinal plant requires the presence of FFA. In the present study, ultrasonic-assisted extraction of FFA from Swertia species was optimized by response surface methodology (RSM), ensuring the highest FFA recoveries. A novel pre-column fluorescence labelling method using 2-(5-benzoacridine)ethyl-p-toluenesulfonate (BAETS) as a labelling reagent has been developed for highly sensitive and selective analysis of FFA by HPLC with fluorescent detection (FLD) and online mass spectrometry identification. RSM was also employed to optimize the fluorescence labelling of FFA. HPLC separation of 17 FFA derivatives was carried out on a reversed-phase Hypersil BDS C-8 column (4.6 x 200 mm, 5 mu m, Agilent Co.) with a gradient elution. The detection limits and quantifications were as low as 0.60 and 1.22 ng mL(-1), respectively. FFA analysis from nine Swertia species was performed by the newly developed method, and the FFA composition of these Swertia samples was first reported.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/25767
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Guoliang,Song, Cuihua,You, Jinmao,et al. Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD[J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, Guoliang Li;Cuihua Song;Jinmao You;Xiaolong Zhang;Guang Chen.Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD,Journal of the American Oil Chemists' Society,2011,28():,2012.
APA	Li, Guoliang,Song, Cuihua,You, Jinmao,Zhang, Xiaolong,&Chen, Guang.(2012).Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD.JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY.
MLA	Li, Guoliang,et al."Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD".JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY (2012).