One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography

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	One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography
	Liu, Yongling ; Wang, Ping ; Chen, Tao ; Jia, Jing ; Sun, Jing ; You, Jinmao ; Li, Yulin
	2014-05-03
发表期刊	SEPARATION SCIENCE AND TECHNOLOGY ; Liu, YL; Wang, P; Chen, T; Jia, J; Sun, J; You, JM; Li, YL.One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography,SEPARATION SCIENCE AND TECHNOLOGY,2014,49(7):1119
摘要	High-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of four xanthone glycosides from Halenia elliptica, a plant widely used in traditional Tibetan medicine. The introduction of HSCCC greatly improved the efficiency of compounds preparation from Halenia elliptica. The following were obtained from 100 mg of crude sample in one-step separation: 2.5 mg of 1-O-primeverosyl-2,3,4,5,7-pentamethoxyxanthone, 7.0 mg of 1-O-primeverosyl-2,3,4,7- tetramethoxyxanthone, 10.0 mg of 1-O-primeverosyl-2,3,5-trimethoxyxanthone (demethyoxyhaleniaside), and 8.5 mg of 1-O-primeverosyl-2,3,4,5-tetramethoxyxanthone. HPLC analysis showed that each target compound had a purity of over 98%, and UV, H-1 NMR, and C-13 NMR data confirmed the component chemical structures.; High-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of four xanthone glycosides from Halenia elliptica, a plant widely used in traditional Tibetan medicine. The introduction of HSCCC greatly improved the efficiency of compounds preparation from Halenia elliptica. The following were obtained from 100 mg of crude sample in one-step separation: 2.5 mg of 1-O-primeverosyl-2,3,4,5,7-pentamethoxyxanthone, 7.0 mg of 1-O-primeverosyl-2,3,4,7- tetramethoxyxanthone, 10.0 mg of 1-O-primeverosyl-2,3,5-trimethoxyxanthone (demethyoxyhaleniaside), and 8.5 mg of 1-O-primeverosyl-2,3,4,5-tetramethoxyxanthone. HPLC analysis showed that each target compound had a purity of over 98%, and UV, H-1 NMR, and C-13 NMR data confirmed the component chemical structures.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/27192
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Liu, Yongling,Wang, Ping,Chen, Tao,et al. One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY, Liu, YL; Wang, P; Chen, T; Jia, J; Sun, J; You, JM; Li, YL.One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography,SEPARATION SCIENCE AND TECHNOLOGY,2014,49(7):1119,2014.
APA	Liu, Yongling.,Wang, Ping.,Chen, Tao.,Jia, Jing.,Sun, Jing.,...&Li, Yulin.(2014).One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography.SEPARATION SCIENCE AND TECHNOLOGY.
MLA	Liu, Yongling,et al."One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography".SEPARATION SCIENCE AND TECHNOLOGY (2014).