Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology

NWIPB OpenIR

	Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology
	Wu, Hongliang ; Li, Guoliang ; Liu, Shucheng ; Ji, Zhongyin ; Zhang, Qiulong ; Hu, Na ; Suo, Yourui ; You, Jinmao ; Li, GL (reprint author), Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China.
	2015-03-01
发表期刊	FOOD ANALYTICAL METHODS
摘要	A simple, sensitive and selective method based on one-step fluorescence labeling and ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) was developed for the determination of biogenic amines (BAs) in foodstuff samples by high-performance liquid chromatography (HPLC) with fluorescence detection (FLD). In this work, fluorescence probe 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) was applied to label BAs. What followed was the UA-DLLME procedure that was carried out using chloroform and acetone as extraction and disperser solvents, respectively. A response surface methodology (RSM) based on a Box-Behnken design (BBD) was employed to optimize the main parameters affecting the fluorescence labeling and DLLME efficiency. Under the optimal conditions, this method offered low limits of detection (LODs) of 1.1-7.8 ng/mL and limits of quantification (LOQs) of 3.5-26.1 ng/mL. Finally, the method was successfully used for the determination of trace BAs in real samples and exhibited powerful potential in the high-throughput sample screening.; A simple, sensitive and selective method based on one-step fluorescence labeling and ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) was developed for the determination of biogenic amines (BAs) in foodstuff samples by high-performance liquid chromatography (HPLC) with fluorescence detection (FLD). In this work, fluorescence probe 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) was applied to label BAs. What followed was the UA-DLLME procedure that was carried out using chloroform and acetone as extraction and disperser solvents, respectively. A response surface methodology (RSM) based on a Box-Behnken design (BBD) was employed to optimize the main parameters affecting the fluorescence labeling and DLLME efficiency. Under the optimal conditions, this method offered low limits of detection (LODs) of 1.1-7.8 ng/mL and limits of quantification (LOQs) of 3.5-26.1 ng/mL. Finally, the method was successfully used for the determination of trace BAs in real samples and exhibited powerful potential in the high-throughput sample screening.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/27425
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wu, Hongliang,Li, Guoliang,Liu, Shucheng,et al. Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology[J]. FOOD ANALYTICAL METHODS,2015.
APA	Wu, Hongliang.,Li, Guoliang.,Liu, Shucheng.,Ji, Zhongyin.,Zhang, Qiulong.,...&Li, GL .(2015).Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology.FOOD ANALYTICAL METHODS.
MLA	Wu, Hongliang,et al."Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology".FOOD ANALYTICAL METHODS (2015).