Isolation and characterization of 15 polymorphic genomic simple sequence repeat markers in Poa pratensis L.

NWIPB OpenIR

	Isolation and characterization of 15 polymorphic genomic simple sequence repeat markers in Poa pratensis L.
	Zhao, Yanyan ; Yu, Feng ; Li, Yuan ; Wang, Haiqing ; Dou, Quanwen
	2015-09-01
发表期刊	GRASSLAND SCIENCE
摘要	Poa pratensis L. is an important perennial grass used both for forage and turf in temperate regions of the world. In this study, we isolated and characterized 15 novel polymorphic genomic simple sequence repeat (SSR) markers in P.pratensis. The polymorphism of each marker was assessed in 14 cultivars. The number of alleles of individual microsatellites ranged from three to six, with an average of 3.8 alleles per SSR. Cross-species amplification tests showed that 13 and seven of the characterized P.pratensis markers could be effectively amplified in P.pratensis var. anceps Gaud and P.crymophila Keng, respectively. These polymorphic microsatellite markers should serve as powerful tools for the investigation of genetic diversity and related analyses, and also for molecular-assisted breeding of P.pratensis.; Poa pratensis L. is an important perennial grass used both for forage and turf in temperate regions of the world. In this study, we isolated and characterized 15 novel polymorphic genomic simple sequence repeat (SSR) markers in P.pratensis. The polymorphism of each marker was assessed in 14 cultivars. The number of alleles of individual microsatellites ranged from three to six, with an average of 3.8 alleles per SSR. Cross-species amplification tests showed that 13 and seven of the characterized P.pratensis markers could be effectively amplified in P.pratensis var. anceps Gaud and P.crymophila Keng, respectively. These polymorphic microsatellite markers should serve as powerful tools for the investigation of genetic diversity and related analyses, and also for molecular-assisted breeding of P.pratensis.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/27491
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhao, Yanyan,Yu, Feng,Li, Yuan,et al. Isolation and characterization of 15 polymorphic genomic simple sequence repeat markers in Poa pratensis L.[J]. GRASSLAND SCIENCE,2015.
APA	Zhao, Yanyan,Yu, Feng,Li, Yuan,Wang, Haiqing,&Dou, Quanwen.(2015).Isolation and characterization of 15 polymorphic genomic simple sequence repeat markers in Poa pratensis L..GRASSLAND SCIENCE.
MLA	Zhao, Yanyan,et al."Isolation and characterization of 15 polymorphic genomic simple sequence repeat markers in Poa pratensis L.".GRASSLAND SCIENCE (2015).