Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat

NWIPB OpenIR

	Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat
	Zhao, D. ; Derkx, A. P. ; Liu, D. -C. ; Buchner, P. ; Hawkesford, M. J.
	2015-07-01
发表期刊	PLANT BIOLOGY
摘要	Increasing the duration of leaf photosynthesis during grain filling using slow-senescing functional stay-green phenotypes is a possible route for increasing grain yields in wheat (Triticum aestivum L.). However, delayed senescence may negatively affect nutrient remobilisation and hence reduce grain protein concentrations and grain quality. A novel NAC1-type transcription factor (hereafter TaNAC-S) was identified in wheat, with gene expression located primarily in leaf/sheath tissues, which decreased during post-anthesis leaf senescence. Expression of TaNAC-S in the second leaf correlated with delayed senescence in two doubled-haploid lines of an AvalonxCadenza population (lines 112 and 181), which were distinct for leaf senescence. Transgenic wheat plants overexpressing TaNAC-S resulted in delayed leaf senescence (stay-green phenotype). Grain yield, aboveground biomass, harvest index and total grain N content were unaffected, but NAC over-expressing lines had higher grain N concentrations at similar grain yields compared to non-transgenic controls. These results indicate that TaNAC-S is a negative regulator of leaf senescence, and that delayed leaf senescence may lead not only to increased grain yields but also to increased grain protein concentrations.; Increasing the duration of leaf photosynthesis during grain filling using slow-senescing functional stay-green phenotypes is a possible route for increasing grain yields in wheat (Triticum aestivum L.). However, delayed senescence may negatively affect nutrient remobilisation and hence reduce grain protein concentrations and grain quality. A novel NAC1-type transcription factor (hereafter TaNAC-S) was identified in wheat, with gene expression located primarily in leaf/sheath tissues, which decreased during post-anthesis leaf senescence. Expression of TaNAC-S in the second leaf correlated with delayed senescence in two doubled-haploid lines of an AvalonxCadenza population (lines 112 and 181), which were distinct for leaf senescence. Transgenic wheat plants overexpressing TaNAC-S resulted in delayed leaf senescence (stay-green phenotype). Grain yield, aboveground biomass, harvest index and total grain N content were unaffected, but NAC over-expressing lines had higher grain N concentrations at similar grain yields compared to non-transgenic controls. These results indicate that TaNAC-S is a negative regulator of leaf senescence, and that delayed leaf senescence may lead not only to increased grain yields but also to increased grain protein concentrations.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/27515
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhao, D.,Derkx, A. P.,Liu, D. -C.,et al. Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat[J]. PLANT BIOLOGY,2015.
APA	Zhao, D.,Derkx, A. P.,Liu, D. -C.,Buchner, P.,&Hawkesford, M. J..(2015).Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat.PLANT BIOLOGY.
MLA	Zhao, D.,et al."Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat".PLANT BIOLOGY (2015).