One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography

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	One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography
	Zhang, Qiulong ; Ma, Tao ; Hu, Na ; Ding, Chenxi ; Liang, Yongxin ; Li, Wencong ; Suo, Yourui ; Ding, Chenxu
	2016-03-01
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
摘要	High-speed counter-current chromatography (CCC) was firstly and successfully applied for the preparative separation and purification of alkaloids from crude extract of Hypecoum leptocarpum. After the measurement of partition coefficient of five target alkaloids in the two-phase solvent systems, the CCC was performed well with a two-phase solvent system composed of tetrachloromethane-chloroform-methanol-0.1 M HCl at a volume ratio of 1.5 : 2.5 : 3 : 2 (V/V/V/V). The upper phase was used as the stationary phase, and the lower phasewas used as the mobile phase. From 120 mg crude extract, 5 mg leptopidine, 32 mg oxohydrastinine, 27 mg (-)-N-methylanadine, 7 mg N-feruloyltyramine and 3 mg hypecoleptopine could be successfully separated. The amides alkaloid, N-feruloyltyramine, was firstly separated from H. leptocarpum. High-performance liquid chromatography analysis showed that the purity of each of the five target alkaloids was over 92%. Their chemical structures were confirmed by H-1-NMR and C-13-NMR data.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/27823
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Qiulong,Ma, Tao,Hu, Na,et al. One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016.
APA	Zhang, Qiulong.,Ma, Tao.,Hu, Na.,Ding, Chenxi.,Liang, Yongxin.,...&Ding, Chenxu.(2016).One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography.JOURNAL OF CHROMATOGRAPHIC SCIENCE.
MLA	Zhang, Qiulong,et al."One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography".JOURNAL OF CHROMATOGRAPHIC SCIENCE (2016).