Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae

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	Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae
	Fang, Zhijia ; Chen, Zhongxiang ; Wang, Song ; Shi, Ping ; Shen, Yuhu ; Zhang, Youshang ; Xiao, Junhua ; Huang, Zhiwei
	2017
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
摘要	The heavy metal cadmium is widely used and released into the environment, posing a severe threat to crops and humans. Saccharomyces cerevisiae is one of the most commonly used organisms in the investigation of environmental metal toxicity. We investigated cadmium stress and the adaptive mechanisms of yeast by screening a genome-wide essential gene overexpression library. A candidate gene, OLE1, encodes a delta-9 desaturase and was associated with high anti-cadmium-stress activity. The results demonstrated that the expression of OLE1 was positively correlated with cadmium stress tolerance and induction was independent of Mga2p and Spt23p (important regulatory factors for OLE1). Moreover, in response to cadmium stress, cellular levels of monounsaturated fatty acids were increased. The addition of exogenous unsaturated fatty acids simulated overexpression of OLE1, leading to cadmium resistance. Such regulation of OLE1 in the synthesis of unsaturated fatty acids may serve as a positive feedback mechanism to help cells counter the lipid peroxidation and cytoplasmic membrane damage caused by cadmium.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/28085
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Fang, Zhijia,Chen, Zhongxiang,Wang, Song,et al. Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017.
APA	Fang, Zhijia.,Chen, Zhongxiang.,Wang, Song.,Shi, Ping.,Shen, Yuhu.,...&Huang, Zhiwei.(2017).Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae.APPLIED AND ENVIRONMENTAL MICROBIOLOGY.
MLA	Fang, Zhijia,et al."Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae".APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2017).