3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification

NWIPB OpenIR

	3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification
	You, Jinmao ; Dou, Kun ; Song, Cuihua ; Li, Guoliang ; Sun, Zhiwei ; Zhang, Shijuan ; Chen, Guang ; Zhao, Xianen ; Hu, Na ; Zhou, Wu
	2017-06-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
摘要	A rapid, sensitive, and selective precolumn derivatization method for the simultaneous determination of eight thiophenols using 3-(2-bromoacetamido)-N-(9-ethyl-9H)-carbazol as a labeling reagent by high-performance liquid chromatography with fluorescence detection has been developed. The labeling reagent reacted with thiophenols at 50 degrees C for 50 min in aqueous acetonitrile in the presence of borate buffer (0.10mol/L, pH 11.2) to give high yields of thiophenol derivatives. The derivatives were identified by online postcolumn mass spectrometry. The collision-induced dissociation spectra for thiophenol derivatives gave the corresponding specific fragment ions at m/z 251.3, 223.3, 210.9, 195.8, and 181.9. At the same time, derivatives exhibited intense fluorescence with an excitation maximum at. ex = 276 nm and an emission maximum at lambda(em) = 385 nm. Excellent linear responses were observed for all analytes over the range of 0.033-6.66 mu mol/L with correlation coefficients of more than 0.9997. Detection limits were in the range of 0.94-5.77 mu g/L with relative standard deviations of less than 4.54%. The feasibility of derivatization allowed the development of a rapid and highly sensitive method for the quantitative analysis of trace levels of thiophenols from some rubber products. The average recoveries (n = 3) were in the range of 87.21-101.12%.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/28249
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	You, Jinmao,Dou, Kun,Song, Cuihua,et al. 3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification[J]. JOURNAL OF SEPARATION SCIENCE,2017.
APA	You, Jinmao.,Dou, Kun.,Song, Cuihua.,Li, Guoliang.,Sun, Zhiwei.,...&Zhou, Wu.(2017).3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification.JOURNAL OF SEPARATION SCIENCE.
MLA	You, Jinmao,et al."3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification".JOURNAL OF SEPARATION SCIENCE (2017).