LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology

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	LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology
	Zhao, Xianen ; Suo, Yourui
	2008-03-01
发表期刊	CHROMATOGRAPHIA ; Zhao Xianen, Suo Yourui.LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology.CHROMATOGRAPHIA,2008,67(5-6):375-382
摘要	An LC method for the determination of 20 amino acids (AAs), using 1,2-Benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as fluorescent labeling reagent, has been validated and applied for the analysis of AAs in rat plasma at three different states concerning exercise physiology. Identification of AA derivatives was carried out by LC-MS with electrospray ion (ESI), and the MS-MS cleavage mode of the representative tyrosine (Tyr) derivative was analyzed. Gradient elution on a Hypersil BDS C-18 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 50-200 mu L of plasma samples. The contents of 20 AAs in rat plasma of three groups (24 rats, group A: quiet state, group B: at exercising exhaust, group C: 12 h after exercising exhaust) exhibited evident difference corresponding to the physiological states. Facile BCEOC derivatization coupled with LC-FLD-ESI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of AAs from plasma or other biochemical samples.; An LC method for the determination of 20 amino acids (AAs), using 1,2-Benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as fluorescent labeling reagent, has been validated and applied for the analysis of AAs in rat plasma at three different states concerning exercise physiology. Identification of AA derivatives was carried out by LC-MS with electrospray ion (ESI), and the MS-MS cleavage mode of the representative tyrosine (Tyr) derivative was analyzed. Gradient elution on a Hypersil BDS C-18 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 50-200 mu L of plasma samples. The contents of 20 AAs in rat plasma of three groups (24 rats, group A: quiet state, group B: at exercising exhaust, group C: 12 h after exercising exhaust) exhibited evident difference corresponding to the physiological states. Facile BCEOC derivatization coupled with LC-FLD-ESI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of AAs from plasma or other biochemical samples.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/30355
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhao, Xianen,Suo, Yourui. LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology[J]. CHROMATOGRAPHIA, Zhao Xianen, Suo Yourui.LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology.CHROMATOGRAPHIA,2008,67(5-6):375-382,2008.
APA	Zhao, Xianen,&Suo, Yourui.(2008).LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology.CHROMATOGRAPHIA.
MLA	Zhao, Xianen,et al."LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology".CHROMATOGRAPHIA (2008).