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Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis | |
Jimenez Joaquin J ; Jy Wenche ; Mauro Lucia M ; Soderland Carl ; Horstman Lawrence L ; Ahn Yeon S | |
2003 | |
发表期刊 | THROMBOSIS RESEARCH
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摘要 | Background: Endothelial cells (EC) shed endothelial microparticles (EMP) in activation and apoptosis. Objectives: We compared the antigenic expression of EMP species released during activation as compared to apoptosis, in three cell lines. Methods: EC from renal and brain microvascular (MiVEC) and coronary macrovascular (MaVEC) origin were incubated with TNF-alpha to induce activation, or deprived of growth factors to induce apoptosis. Antigens expressed on EMP and EC were assayed flow cytometrically and included constitutive markers (CD31, CD51/61, CD105), inducible markers (CD54, CD62E and CD106), and annexin V binding. Results: It was found that in apoptosis, constitutive markers in EMP were markedly increased (CD31>CD105), with a concomitant decrease in expression in EC. Annexin V EC surface binding and annexin V+ EMP were more sharply increased in apoptosis than in activation. In contrast, in activation, inducible markers in EMP were markedly increased in both EMP and EC (CD62E>CD54>CD 106). Coronary MaVEC released significantly less EMP than MiVEC. Conclusion: EC release qualitatively and quantitatively distinct EMP during activation compared to apoptosis. Analysis of EMP phenotypic signatures may provide clinically useful information on the status of the endothelium. (C) 2003 Elsevier Science Ltd. All rights reserved. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/30533 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Jimenez Joaquin J,Jy Wenche,Mauro Lucia M,et al. Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis[J]. THROMBOSIS RESEARCH, Jimenez Joaquin J, Jy Wenche, Mauro Lucia M, Soderland Carl, Horstman Lawrence L, Ahn Yeon S.Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis.THROMBOSIS RESEARCH,2003,109(4):175-180,2003. |
APA | Jimenez Joaquin J,Jy Wenche,Mauro Lucia M,Soderland Carl,Horstman Lawrence L,&Ahn Yeon S.(2003).Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis.THROMBOSIS RESEARCH. |
MLA | Jimenez Joaquin J,et al."Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis".THROMBOSIS RESEARCH (2003). |
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