Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis | |
| LU Ting; Edward P C Lai; Jorn C C Yu; HU Fengzu | |
| 2006 | |
| 发表期刊 | 食品科学
 |
| 卷号 | 27期号:12页码:582-587 |
| 摘要 | A capillary electrophoresis (CE) method has been developed for the determination of six bioactive flavonoids that are commonly found in health foods: hesperidin, hyperin, isorhamnetin, kaempferol, quercetin and rutin. The effects of several parameters, such as pH, buffer concentration, separation voltage and UV detector wavelength, were investigated to find the optimal conditions. Using a HbBCh-NaiB-iO? buffer (pH 9.2), the analytes can be separated within 8 min. The relative standard deviations of migration times in eight injections were between 0.77% and 0.93%, and those of the peak areas ranged from 3.8% to 8.6%. A high reproducibility and excellent linearity was observed over two orders of magnitude, with detection limits (S/N = 3) ranging from 0.34ug ml to 2.9ug ml for all the six analytes. Recoveries ranged from 80.4 % to 113.9 %. The new method is simple, reproducible and sensitive. No solid phase extraction for sample pretreatment is necessary. Analysis results are accurate in application to bee pollens.; A capillary electrophoresis (CE) method has been developed for the determination of six bioactive flavonoids that are commonly found in health foods: hesperidin, hyperin, isorhamnetin, kaempferol, quercetin and rutin. The effects of several parameters, such as pH, buffer concentration, separation voltage and UV detector wavelength, were investigated to find the optimal conditions. Using a HbBCh-NaiB-iO? buffer (pH 9.2), the analytes can be separated within 8 min. The relative standard deviations of migration times in eight injections were between 0.77% and 0.93%, and those of the peak areas ranged from 3.8% to 8.6%. A high reproducibility and excellent linearity was observed over two orders of magnitude, with detection limits (S/N = 3) ranging from 0.34ug ml to 2.9ug ml for all the six analytes. Recoveries ranged from 80.4 % to 113.9 %. The new method is simple, reproducible and sensitive. No solid phase extraction for sample pretreatment is necessary. Analysis results are accurate in application to bee pollens. |
| 关键词 | 分析 黄酮类化合物 蜂花粉 毛细管电泳 |
| 学科领域 | 生物科学 |
| 收录类别 | CSCD |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2625250 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/306 |
| 专题 | 中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | LU Ting,Edward P C Lai,Jorn C C Yu,et al. Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis[J]. 食品科学,2006,27(12):582-587. |
| APA | LU Ting,Edward P C Lai,Jorn C C Yu,&HU Fengzu.(2006).Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis.食品科学,27(12),582-587. |
| MLA | LU Ting,et al."Analysis for Flavonoids in Bee Pollens by Capillary Electrophoresis".食品科学 27.12(2006):582-587. |
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