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2-(5-Benzoacridine)ethyl-p-toluenesulfonate as sensitive reagent for the determination of bile acids by HPLC with fluorescence detection and online atmospheric chemical ionization-mass spectrometric identification | |
You, Jinmao ; Fu, Yanyan ; Sun, Zhiwei ; Suo, Yourui | |
2010-04-01 | |
发表期刊 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY ; You, JM; Fu, YY; Sun, ZW; Suo, YR.2-(5-Benzoacridine)ethyl-p-toluenesulfonate as sensitive reagent for the determination of bile acids by HPLC with fluorescence detection and online atmospheric chemical ionization-mass spectrometric identification,ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2010,396(7):2657-2666 |
摘要 | 2-(5-Benzoacridine)ethyl-p-toluenesulfonate (BAETS), a dual-sensitive probe, was reacted with bile acids in the presence of K(2)CO(3) catalyst in dimethyl sulfoxide (DMSO) solvent to give BAETS-bile acid derivatives. Derivatives exhibited intense fluorescence (FL) with an excitation maximum lambda(ex) 270 nm and an emission maximum lambda(em) 510 nm. MS analysis using APCI-MS indicated that derivatives had excellent APCI-MS ionizability with percentage ionization delta values changing from 0 to 88.83% in aqueous acetonitrile and from 0 to 89.15% in aqueous methanol. The collision induced dissociation spectra of m/z [M + H](+) contained specific fragment ions at m/z [M + H-H(2)O](+), [M + H-2H(2)O](+), [M + H-3H(2)O](+), 347.3, and 290.1. Repeatability was good for LC separation of BAETS-bile acid derivatives with aqueous acetonitrile as mobile phase. The relative standard deviations ( RSDs) of retention time and peak area at 6.6 nmol mL(-1) levels with fluorescence detection (FL) were from 0.045 to 0.072% and from 2.16 to 2.73%, respectively. Excellent linear responses were observed, with regression coefficients >0.9995. The FL detection limits (S/N=3) were in the range of 18.0-36.1 fmol. The online APCI-MS detection limits are in the range of 500-790 fmol (at a signal-to-noise ratio of 3).; 2-(5-Benzoacridine)ethyl-p-toluenesulfonate (BAETS), a dual-sensitive probe, was reacted with bile acids in the presence of K(2)CO(3) catalyst in dimethyl sulfoxide (DMSO) solvent to give BAETS-bile acid derivatives. Derivatives exhibited intense fluorescence (FL) with an excitation maximum lambda(ex) 270 nm and an emission maximum lambda(em) 510 nm. MS analysis using APCI-MS indicated that derivatives had excellent APCI-MS ionizability with percentage ionization delta values changing from 0 to 88.83% in aqueous acetonitrile and from 0 to 89.15% in aqueous methanol. The collision induced dissociation spectra of m/z [M + H](+) contained specific fragment ions at m/z [M + H-H(2)O](+), [M + H-2H(2)O](+), [M + H-3H(2)O](+), 347.3, and 290.1. Repeatability was good for LC separation of BAETS-bile acid derivatives with aqueous acetonitrile as mobile phase. The relative standard deviations ( RSDs) of retention time and peak area at 6.6 nmol mL(-1) levels with fluorescence detection (FL) were from 0.045 to 0.072% and from 2.16 to 2.73%, respectively. Excellent linear responses were observed, with regression coefficients >0.9995. The FL detection limits (S/N=3) were in the range of 18.0-36.1 fmol. The online APCI-MS detection limits are in the range of 500-790 fmol (at a signal-to-noise ratio of 3). |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/30731 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | You, Jinmao,Fu, Yanyan,Sun, Zhiwei,et al. 2-(5-Benzoacridine)ethyl-p-toluenesulfonate as sensitive reagent for the determination of bile acids by HPLC with fluorescence detection and online atmospheric chemical ionization-mass spectrometric identification[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, You, JM; Fu, YY; Sun, ZW; Suo, YR.2-(5-Benzoacridine)ethyl-p-toluenesulfonate as sensitive reagent for the determination of bile acids by HPLC with fluorescence detection and online atmospheric chemical ionization-mass spectrometric identification,ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2010,396(7):2657-2666,2010. |
APA | You, Jinmao,Fu, Yanyan,Sun, Zhiwei,&Suo, Yourui.(2010).2-(5-Benzoacridine)ethyl-p-toluenesulfonate as sensitive reagent for the determination of bile acids by HPLC with fluorescence detection and online atmospheric chemical ionization-mass spectrometric identification.ANALYTICAL AND BIOANALYTICAL CHEMISTRY. |
MLA | You, Jinmao,et al."2-(5-Benzoacridine)ethyl-p-toluenesulfonate as sensitive reagent for the determination of bile acids by HPLC with fluorescence detection and online atmospheric chemical ionization-mass spectrometric identification".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2010). |
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