Syntheses of Fluorescence Probe 5-[2-(1H-imlidazol-1-yl)-2-oxoethyl] benzo[b]acridin-12(5H)-one and Its Application for the Determination of Aliphatic Amines with LC-APCI-MS

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	Syntheses of Fluorescence Probe 5-[2-(1H-imlidazol-1-yl)-2-oxoethyl] benzo[b]acridin-12(5H)-one and Its Application for the Determination of Aliphatic Amines with LC-APCI-MS
	Fu Yan-Yan ; Sun Zhi-Wei ; Zhao Huai-Xin ; Bai Xin-Wei ; Suo You-Rui ; Li Yu-Lin ; You Jin-Mao
	2009-11-10
发表期刊	CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE ; Fu, YY; Sun, ZW; Zhao, HX; Bai, XW; Suo, YR; Li, YL; You, JM.Syntheses of Fluorescence Probe 5-[2-(1H-imlidazol-1-yl)-2-oxoethyl] benzo[b]acridin-12(5H)-one and Its Application for the Determination of Aliphatic Amines with LC-APCI-MS,CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2009,30(11):2146-2153
摘要	2-{12-Oxobenzo[b] acridin-5(12H)-yl} -acetic acid (BAAA) reacted with coupling agent N, N'-carbonyldiimidazole (CDI) to form an activated amide intermediate 5-[2-(1H-imidazol-1-yl)-2-oxoethyl] benzo[b]acridin-12(5H)-one(IEBA), which was a novel fluorescence probe. The amide intermediate(IEBA) reacted preferably with amines in DMF solvent in the absence of catalysts to give the high yields of derivatives, which not only have high fluorescence sensitivity but also have strong ionizable ability. The optimum excitation and emission wavelengths were at lambda(ex)/lambda(em) = 272 nm/505 nm. The percent ionization 8 values in aqueous acetonitrile and aqueous methanol were in the range of 0-57. 32% and 0-62. 14%, respectively. The fluorescence detection limits of twelve amine derivatives(at a signal-to-noise ratio of 3) were in the range of 0. 15-0. 50 ng/mL. The online APCI-MS detection limits were at levels of 1. 43-8. 51 ng/mL(S/N = 3).; 2-{12-Oxobenzo[b] acridin-5(12H)-yl} -acetic acid (BAAA) reacted with coupling agent N, N'-carbonyldiimidazole (CDI) to form an activated amide intermediate 5-[2-(1H-imidazol-1-yl)-2-oxoethyl] benzo[b]acridin-12(5H)-one(IEBA), which was a novel fluorescence probe. The amide intermediate(IEBA) reacted preferably with amines in DMF solvent in the absence of catalysts to give the high yields of derivatives, which not only have high fluorescence sensitivity but also have strong ionizable ability. The optimum excitation and emission wavelengths were at lambda(ex)/lambda(em) = 272 nm/505 nm. The percent ionization 8 values in aqueous acetonitrile and aqueous methanol were in the range of 0-57. 32% and 0-62. 14%, respectively. The fluorescence detection limits of twelve amine derivatives(at a signal-to-noise ratio of 3) were in the range of 0. 15-0. 50 ng/mL. The online APCI-MS detection limits were at levels of 1. 43-8. 51 ng/mL(S/N = 3).
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/30771
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Fu Yan-Yan,Sun Zhi-Wei,Zhao Huai-Xin,et al. Syntheses of Fluorescence Probe 5-[2-(1H-imlidazol-1-yl)-2-oxoethyl] benzo[b]acridin-12(5H)-one and Its Application for the Determination of Aliphatic Amines with LC-APCI-MS[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, Fu, YY; Sun, ZW; Zhao, HX; Bai, XW; Suo, YR; Li, YL; You, JM.Syntheses of Fluorescence Probe 5-[2-(1H-imlidazol-1-yl)-2-oxoethyl] benzo[b]acridin-12(5H)-one and Its Application for the Determination of Aliphatic Amines with LC-APCI-MS,CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2009,30(11):2146-2153,2009.
APA	Fu Yan-Yan.,Sun Zhi-Wei.,Zhao Huai-Xin.,Bai Xin-Wei.,Suo You-Rui.,...&You Jin-Mao.(2009).Syntheses of Fluorescence Probe 5-[2-(1H-imlidazol-1-yl)-2-oxoethyl] benzo[b]acridin-12(5H)-one and Its Application for the Determination of Aliphatic Amines with LC-APCI-MS.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE.
MLA	Fu Yan-Yan,et al."Syntheses of Fluorescence Probe 5-[2-(1H-imlidazol-1-yl)-2-oxoethyl] benzo[b]acridin-12(5H)-one and Its Application for the Determination of Aliphatic Amines with LC-APCI-MS".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE (2009).