Isolation, analysis and prokaryotic expression of nitric oxide associated factor gene-NOA1 from potato

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	Isolation, analysis and prokaryotic expression of nitric oxide associated factor gene-NOA1 from potato
	Zhang HG(张怀刚)
	2011
发表期刊	African Journal of Biotechnology ; 张怀刚.Isolation, analysis and prokaryotic expression of nitric oxide associated factor gene-NOA1 from potato,African Journal of Biotechnology,2011,9(18):2606-2612
摘要	In this study, the cDNA of a NOA (nitric oxide associated factor) was isolated with the strategy of EST in silico cloning in combination with RT-PCR and 3’RACE from potato (Solanum tuberosum L.) for the first time (named StNOA1). The StNOA1 bears a centrally positioned GTPase-binding domain. Sequence alignment and phylogenetic analysis of the deduced StNOA1 protein with other known NOA family protein indicates that StNOA1 is highly homologenous with NbNOA1 (NOA from Nicotiana benthamiana). The cDNA was cloned into prokaryotic expression vector, pET-30a (+) and expressed in Escherichia coli BL21 (DE3) after induction with IPTG. The recombinant protein was dissolved by 8 M urea and recovered by dialysis due to most of them were in inclusion bodies. Then the recovered recombinant protein was purified by Ni-NTA and analyzed by SDS-PAGE. The results of SDS-PAGE showed that the StNOA1 was successfully expressed with the pET prokaryotic expression system and purified. The present study is the basis for further elucidating the biochemical characteristics of StNOA1 and is very significant for elucidating the nature of plant NOA and its action mechanisms in endogenous NO synthesis in plant species.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/30934
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang HG. Isolation, analysis and prokaryotic expression of nitric oxide associated factor gene-NOA1 from potato[J]. African Journal of Biotechnology, 张怀刚.Isolation, analysis and prokaryotic expression of nitric oxide associated factor gene-NOA1 from potato,African Journal of Biotechnology,2011,9(18):2606-2612,2011.
APA	张怀刚.(2011).Isolation, analysis and prokaryotic expression of nitric oxide associated factor gene-NOA1 from potato.African Journal of Biotechnology.
MLA	张怀刚."Isolation, analysis and prokaryotic expression of nitric oxide associated factor gene-NOA1 from potato".African Journal of Biotechnology (2011).