LC Determination of Trace Biogenic Amines in Foods Samples

NWIPB OpenIR

	LC Determination of Trace Biogenic Amines in Foods Samples
	Li Kang ; Jinmao You ; Zhiwei Sun ; Chengyan Wang ; Zhongyin Ji ; Yongping Gao ; Yourui Suo ; Yulin Li
	2011-01-08
发表期刊	Chromatographia ; Li Kang;Jinmao You;Zhiwei Sun;Chengyan Wang;Zhongyin Ji;Yongping Gao;Yourui Suo;Yulin Li.LC Determination of Trace Biogenic Amines in Foods Samples,Chromatographia,2011,73():43-50
摘要	A pre-column derivatization method for the simple, sensitive determination of biogenic amines using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as labeling reagent with fluorescence detection and mass spectrometry (MS) identification has been developed. After pre-column derivatization, the labeled biogenic amines were separated on a Hypersil BDS-C18 column by gradient elution. The derivatives showed an intense protonated molecular ion corresponding m/z [M ? H]? in positive-ion mode. The collision-induced dissociation of protonated molecular ion formed specific fragment ions at m/z 196.5, m/z 222.7, m/z 224.4 and m/z 272.5, m/z 286.2. Satisfactory linear responses were observed at the concentration range of 0.02–10 lmol L-1 with coefficients of[0.9993. Detection limits obtained by the analysis of a derivatized standard containing 0.2 pmol of each biogenic amine, were from 20.22 to 109.2 fmol (at a signal-to-noise ratio of 3). The relative standard deviations of retention times and peak areas for each biogenic amine were \0.96 and 3.22%, respectively. Recoveries except for PUT were in the range of 96.7–103.6% for chicken sausage and 95.8–104.6% for pork sausage The established method for the determination of biogenic amines except for PUT from real samples was satisfactory.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/30937
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li Kang,Jinmao You,Zhiwei Sun,et al. LC Determination of Trace Biogenic Amines in Foods Samples[J]. Chromatographia, Li Kang;Jinmao You;Zhiwei Sun;Chengyan Wang;Zhongyin Ji;Yongping Gao;Yourui Suo;Yulin Li.LC Determination of Trace Biogenic Amines in Foods Samples,Chromatographia,2011,73():43-50,2011.
APA	Li Kang.,Jinmao You.,Zhiwei Sun.,Chengyan Wang.,Zhongyin Ji.,...&Yulin Li.(2011).LC Determination of Trace Biogenic Amines in Foods Samples.Chromatographia.
MLA	Li Kang,et al."LC Determination of Trace Biogenic Amines in Foods Samples".Chromatographia (2011).