One-step Preparative Separation of Two Polyhydroxystilbenes from Rheum likiangense Sam. by High-speed Counter-current Chromatography

NWIPB OpenIR

	One-step Preparative Separation of Two Polyhydroxystilbenes from Rheum likiangense Sam. by High-speed Counter-current Chromatography
	Wang, Xiaoyan ; Wang, Honglun ; Ding, Chenxu ; Suo, Yourui
	2012-11-01
发表期刊	PHYTOCHEMICAL ANALYSIS ; Chen, SY; Liu, WJ; Qin, X; Liu, YS; Zhang, TZ; Chen, KL; Hu, FZ; Ren, JW; Qin, DH.One-step Preparative Separation of Two Polyhydroxystilbenes from Rheum likiangense Sam. by High-speed Counter-current Chromatography,PHYTOCHEMICAL ANALYSIS,2012,23(6):684-688
摘要	Introduction The official rhubarb species have frequently been investigated for their hydroxyanthraquinone components and associated pharmacological properties. However, other unofficial rhubarb species were rarely studied until polyhydroxystilbenes (PHS), which commonly occur in the unofficial rhubarb, revealed a range of potential bioactivities. Hence, there has been increasing interest in the efficient preparation of high-purity PHS for pharmacological and clinical trials. Objective To develop a suitable method for large-scale preparative separation of PHS from the rhizome of Rheum likiangense Sam. by high-speed counter-current chromatography (HSCCC). Results Two PHS compounds were isolated successfully within 440 min using a solvent system consisting of methanol:n-butanol:chloroform:water (2:0.5:3:3, v/v/v/v). Eighty-four milligrams of desoxyrhaponticin with 98.2% purity and 148 mg of rhaponticin with 95.3% purity were respectively yielded from 1.5?g of crude extract in the single, one-step operation. Conclusion An optimised HSCCC method has been established for large-scale preparative separation of PHS compounds from Rheum likiangense Sam. Copyright (c) 2012 John Wiley & Sons, Ltd.; Introduction The official rhubarb species have frequently been investigated for their hydroxyanthraquinone components and associated pharmacological properties. However, other unofficial rhubarb species were rarely studied until polyhydroxystilbenes (PHS), which commonly occur in the unofficial rhubarb, revealed a range of potential bioactivities. Hence, there has been increasing interest in the efficient preparation of high-purity PHS for pharmacological and clinical trials. Objective To develop a suitable method for large-scale preparative separation of PHS from the rhizome of Rheum likiangense Sam. by high-speed counter-current chromatography (HSCCC). Results Two PHS compounds were isolated successfully within 440 min using a solvent system consisting of methanol:n-butanol:chloroform:water (2:0.5:3:3, v/v/v/v). Eighty-four milligrams of desoxyrhaponticin with 98.2% purity and 148 mg of rhaponticin with 95.3% purity were respectively yielded from 1.5?g of crude extract in the single, one-step operation. Conclusion An optimised HSCCC method has been established for large-scale preparative separation of PHS compounds from Rheum likiangense Sam. Copyright (c) 2012 John Wiley & Sons, Ltd.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/31748
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Xiaoyan,Wang, Honglun,Ding, Chenxu,et al. One-step Preparative Separation of Two Polyhydroxystilbenes from Rheum likiangense Sam. by High-speed Counter-current Chromatography[J]. PHYTOCHEMICAL ANALYSIS, Chen, SY; Liu, WJ; Qin, X; Liu, YS; Zhang, TZ; Chen, KL; Hu, FZ; Ren, JW; Qin, DH.One-step Preparative Separation of Two Polyhydroxystilbenes from Rheum likiangense Sam. by High-speed Counter-current Chromatography,PHYTOCHEMICAL ANALYSIS,2012,23(6):684-688,2012.
APA	Wang, Xiaoyan,Wang, Honglun,Ding, Chenxu,&Suo, Yourui.(2012).One-step Preparative Separation of Two Polyhydroxystilbenes from Rheum likiangense Sam. by High-speed Counter-current Chromatography.PHYTOCHEMICAL ANALYSIS.
MLA	Wang, Xiaoyan,et al."One-step Preparative Separation of Two Polyhydroxystilbenes from Rheum likiangense Sam. by High-speed Counter-current Chromatography".PHYTOCHEMICAL ANALYSIS (2012).