Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography

NWIPB OpenIR

	Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography
	Jia, Jing ; Chen, Tao ; Wang, Ping ; Chen, Guichen ; You, Jinmao ; Liu, Yongjun ; Li, Yulin
	2012-07-01
发表期刊	PHYTOCHEMICAL ANALYSIS ; Zhang, SJ; You, JM; Sun, ZW; Song, CH; Ning, SJ; Zhao, CS; Suo, YR.Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography,PHYTOCHEMICAL ANALYSIS,2012,23(4):332-336
摘要	Introduction Xanthones, the primary constituents of Swertia mussotii, are known to possess a variety of biological activities, including anti-depressant, anti-leukaemic, anti-tumour, anti-tubercular, choleretic, diuretic, anti-microbial, anti-fungal, anti-inflammatory, anti-viral, cardiotonic and hypoglycemic properties. However, high performance, environmentally friendly methods for isolating and purifying xanthones from S. mussotii are not currently available. Objective To develop a high performance and environmentally friendly method for the preparative separation of xanthones methylswertianin, swerchirin and decussatin from S. mussotii using high-speed counter-current chromatography (HSCCC). Methodology A solvent system composed of n-hexane:ethyl acatate:methanol:water (5:5:10:4, v/v/v/v) was developed for the separation method. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5?mL/min, a rotation speed of 800?rpm and a temperature of 25 degrees C. Results Using the described method, 8?mg of methylswertianin, 21?mg of swerchirin and 11?mg of decussatin with purities of over 98% could be isolated from a 150?mg crude sample. They were identified by 1H-NMR and 13?C-NMR analysis. Conclusion Three xanthones in Swertia mussotii could be systematically isolated and purified using HSCCC. Copyright (C) 2011 John Wiley & Sons, Ltd.; Introduction Xanthones, the primary constituents of Swertia mussotii, are known to possess a variety of biological activities, including anti-depressant, anti-leukaemic, anti-tumour, anti-tubercular, choleretic, diuretic, anti-microbial, anti-fungal, anti-inflammatory, anti-viral, cardiotonic and hypoglycemic properties. However, high performance, environmentally friendly methods for isolating and purifying xanthones from S. mussotii are not currently available. Objective To develop a high performance and environmentally friendly method for the preparative separation of xanthones methylswertianin, swerchirin and decussatin from S. mussotii using high-speed counter-current chromatography (HSCCC). Methodology A solvent system composed of n-hexane:ethyl acatate:methanol:water (5:5:10:4, v/v/v/v) was developed for the separation method. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5?mL/min, a rotation speed of 800?rpm and a temperature of 25 degrees C. Results Using the described method, 8?mg of methylswertianin, 21?mg of swerchirin and 11?mg of decussatin with purities of over 98% could be isolated from a 150?mg crude sample. They were identified by 1H-NMR and 13?C-NMR analysis. Conclusion Three xanthones in Swertia mussotii could be systematically isolated and purified using HSCCC. Copyright (C) 2011 John Wiley & Sons, Ltd.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/31776
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Jia, Jing,Chen, Tao,Wang, Ping,et al. Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography[J]. PHYTOCHEMICAL ANALYSIS, Zhang, SJ; You, JM; Sun, ZW; Song, CH; Ning, SJ; Zhao, CS; Suo, YR.Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography,PHYTOCHEMICAL ANALYSIS,2012,23(4):332-336,2012.
APA	Jia, Jing.,Chen, Tao.,Wang, Ping.,Chen, Guichen.,You, Jinmao.,...&Li, Yulin.(2012).Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography.PHYTOCHEMICAL ANALYSIS.
MLA	Jia, Jing,et al."Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography".PHYTOCHEMICAL ANALYSIS (2012).