NWIPB OpenIR
拟南芥Atfin1锌指蛋白基因的功能研究
其他题名Function analysis of Atfin1 in Arabidopsis thaliana.
王沛
学位类型硕士
导师王海庆
2005-06-04
学位授予单位中国科学院西北高原生物研究所
学位授予地点西北高原生物研究所
关键词拟南芥 锌指蛋白 盐胁迫 细胞定位
摘要盐碱、干旱等非生物逆境是影响植物生长发育的主要环境因素,严重地制约着农作物产量与品质的提高。在逆境胁迫下,植物能感知、传递胁迫信号,通过激活响应转录因子,启动抗逆功能基因的表达,使植物体内发生一系列的生理生化反应,抵御不良环境的危害,从而提高植物的耐逆性。 转录调控是植物生长发育、逆境应答、信号转导等一系列基因表达的最主要调控形式,转录因子是参与基因转录水平调控过程的重要反式作用因子。Alfin1 是在紫花苜蓿中发现的具有Cys4-His-Cys3锌指结构特征的与耐盐胁迫相关的转录因子。拟南芥基因组中有7个基因所编码的氨基酸序列和Alfin1 具有同源性。序列分析发现,由At2g02470和At1g14510(Atfin1)所编码的氨基酸序列与Alfin1的同源性最高。Northern杂交分析显示,Atfin1表达受NaCl(300mmol/L)胁迫诱导,并随着胁迫时间的延长表现为上调。将Atfin1和绿色荧光蛋白(EGFP)融合,在烟草叶片中进行瞬间表达,发现融合蛋白定位于细胞核中,符合作为转录因子的细胞分布特点。Atfin1过量表达的转基因拟南芥幼苗主根变短,侧根数增多;在盐胁迫情况下,表现出具一定的耐盐性。因此拟南芥Atfin1锌指蛋白在根系发育和耐盐信号转导途径中具有和紫花苜蓿Alfin1锌指结构蛋白类似的功能。
其他摘要Salinity and drought are the key environment factors affecting plant growth and development, and seriously limit the increase of yield and improvement of quality of crops. Under abiotic stresses, plants could sense and transduce the signals caused by stresses to transcription factors through a series of pathways The functional genes related to stress tolerance are then initiated; a series of physiochemical reactions are activated to alleviate the damage caused by abiotic stress, finally the stress tolerance of plants is enhanced. As were shown in numerous studies, transcription control is the most important adjustment ways of the genes expressed in plants during growth, development and response to stresses. Transcription factors are the important trans-acting factor involved in the control of transcription. Alfin1 is a novel transcription factor with a Cys4-His-Cys3 zinc finger motif, associated with salty tolerance in Medicago sativa.There are seven genes encoding proteins for Cys4-His-Cys3 zinc finger-type proteins in Arabidopsis genome with homology to Alfin1. Alignment and phylogenetic analysis indicated that At2g02470 and At1g14510 showed the highest amino acid sequence identity with Alfin1. Northern blotting showed that Atfin1 is up-regulated by NaCl (300mmol/L) temporally. Atfin1-EGFP fusion was localized in nucleus by means of transient expression in tobacco leaf epidermis. Transgenic overexpression of Atfin1 increased number of lateral root while the length of main root was reduced in Arabidopsis seedlings. Under salinity stress condition, overexpression of Atfin1 improved the salinity tolerance of the Arabidopsis seedlings. Therefore, the Alfin1-like transcription factors maybe play universal roles in plant root development and response to salinity stress.
页数68
语种中文
文献类型学位论文
条目标识符http://210.75.249.4/handle/363003/3178
专题中国科学院西北高原生物研究所
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王沛. 拟南芥Atfin1锌指蛋白基因的功能研究[D]. 西北高原生物研究所. 中国科学院西北高原生物研究所,2005.
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