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Optimization of Microwave-Assisted Extraction of Cordycepic Acid and Cordycepin from Cultured Cordyceps militaris by Response Surface Methodology
Deng, Li ; Zhou, Tong-Yong ; Pi, Li ; Zhao, Xiao-Hui ; Han, Tao ; Li, Yi-Kang ; Han, Fa ; Han, F (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Peoples R China.
2013-10-01
发表期刊ASIAN JOURNAL OF CHEMISTRY ; Deng, L; Zhou, TY; Pi, L; Zhao, XH; Han, T; Li, YK; Han, F.Optimization of Microwave-Assisted Extraction of Cordycepic Acid and Cordycepin from Cultured Cordyceps militaris by Response Surface Methodology,ASIAN JOURNAL OF CHEMISTRY,2013,25(14):8065-8071
摘要The response surface methodology was employed to optimize the integrated extraction parameters of cordycepic acid and cordycepin from cultured Cordyceps militaris (L.) link based on a single-factor experiment. The Box-Behnken design with three independent variables i.e., microwave power (W), water/material ratio (mL/g) and extraction time (min) was used. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis. The three-dimensional response surface plot and contour plot derived from the mathematical models were applied to determine the optimal conditions. The optimum extraction condition was obtained as follows: microwave-assisted extraction, microwave power of 649.33 W, extraction time of 5.74 min, water/material ratio of 38. 99 mL/g and extraction number of three. The yield of cordycepic acid and cordycepin were 2.47 and 0.79 %, respectively. Under these conditions, the experimental values of 3.12 and 0.75 % well agreed with those predicted by the model.; The response surface methodology was employed to optimize the integrated extraction parameters of cordycepic acid and cordycepin from cultured Cordyceps militaris (L.) link based on a single-factor experiment. The Box-Behnken design with three independent variables i.e., microwave power (W), water/material ratio (mL/g) and extraction time (min) was used. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis. The three-dimensional response surface plot and contour plot derived from the mathematical models were applied to determine the optimal conditions. The optimum extraction condition was obtained as follows: microwave-assisted extraction, microwave power of 649.33 W, extraction time of 5.74 min, water/material ratio of 38. 99 mL/g and extraction number of three. The yield of cordycepic acid and cordycepin were 2.47 and 0.79 %, respectively. Under these conditions, the experimental values of 3.12 and 0.75 % well agreed with those predicted by the model.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/31978
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Deng, Li,Zhou, Tong-Yong,Pi, Li,et al. Optimization of Microwave-Assisted Extraction of Cordycepic Acid and Cordycepin from Cultured Cordyceps militaris by Response Surface Methodology[J]. ASIAN JOURNAL OF CHEMISTRY, Deng, L; Zhou, TY; Pi, L; Zhao, XH; Han, T; Li, YK; Han, F.Optimization of Microwave-Assisted Extraction of Cordycepic Acid and Cordycepin from Cultured Cordyceps militaris by Response Surface Methodology,ASIAN JOURNAL OF CHEMISTRY,2013,25(14):8065-8071,2013.
APA Deng, Li.,Zhou, Tong-Yong.,Pi, Li.,Zhao, Xiao-Hui.,Han, Tao.,...&Han, F .(2013).Optimization of Microwave-Assisted Extraction of Cordycepic Acid and Cordycepin from Cultured Cordyceps militaris by Response Surface Methodology.ASIAN JOURNAL OF CHEMISTRY.
MLA Deng, Li,et al."Optimization of Microwave-Assisted Extraction of Cordycepic Acid and Cordycepin from Cultured Cordyceps militaris by Response Surface Methodology".ASIAN JOURNAL OF CHEMISTRY (2013).
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