Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection

NWIPB OpenIR

	Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection
	Li, Yanxin ; Zhang, Shijuan ; Song, Cuihua ; You, Jinmao ; You, JM (reprint author), Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Peoples R China.
	2013-10-01
发表期刊	FOOD ANALYTICAL METHODS ; Li, YX; Zhang, SJ; Song, CH; You, JM.Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection,FOOD ANALYTICAL METHODS,2013,6(5):1284-1290
摘要	A highly sensitive and selective method was developed for the purification and determination of bisphenol A and alkylphenols in soft drinks by using 2-(11H-benzo[a]carbazol-11-yl) ethyl chloroformate (BCEC-Cl) as pre-column labeling reagent followed by high-performance liquid chromatography (HPLC) with fluorescence detection. The HPLC sensitivity of bisphenol A and alkylphenols was greatly enhanced through the introduction of BCEC-Cl moiety with excellent fluorescence property into the target molecules. Meanwhile, the introduction of highly hydrophobic BCEC-Cl moiety into the analytes also greatly increased the hydrophobicity of the target compounds and distinguished them from hydrophilic matrices. Therefore, little interference was observed. Solid-phase extraction with C18 cartridges was applied to sample purification procedure with recoveries of higher than 82 %. When 20 mL of sample was used for analysis, the limits of quantifications of the analytes were between 0.06 and 0.1 mu g L-1. The proposed method was successfully applied to the determination of the target compounds in soft drink samples with a much higher sensitivity than traditional HPLC methods.; A highly sensitive and selective method was developed for the purification and determination of bisphenol A and alkylphenols in soft drinks by using 2-(11H-benzo[a]carbazol-11-yl) ethyl chloroformate (BCEC-Cl) as pre-column labeling reagent followed by high-performance liquid chromatography (HPLC) with fluorescence detection. The HPLC sensitivity of bisphenol A and alkylphenols was greatly enhanced through the introduction of BCEC-Cl moiety with excellent fluorescence property into the target molecules. Meanwhile, the introduction of highly hydrophobic BCEC-Cl moiety into the analytes also greatly increased the hydrophobicity of the target compounds and distinguished them from hydrophilic matrices. Therefore, little interference was observed. Solid-phase extraction with C18 cartridges was applied to sample purification procedure with recoveries of higher than 82 %. When 20 mL of sample was used for analysis, the limits of quantifications of the analytes were between 0.06 and 0.1 mu g L-1. The proposed method was successfully applied to the determination of the target compounds in soft drink samples with a much higher sensitivity than traditional HPLC methods.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/31983
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Yanxin,Zhang, Shijuan,Song, Cuihua,et al. Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection[J]. FOOD ANALYTICAL METHODS, Li, YX; Zhang, SJ; Song, CH; You, JM.Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection,FOOD ANALYTICAL METHODS,2013,6(5):1284-1290,2013.
APA	Li, Yanxin,Zhang, Shijuan,Song, Cuihua,You, Jinmao,&You, JM .(2013).Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection.FOOD ANALYTICAL METHODS.
MLA	Li, Yanxin,et al."Determination of Bisphenol A and Alkylphenols in Soft Drinks by High-Performance Liquid Chromatography with Fluorescence Detection".FOOD ANALYTICAL METHODS (2013).