Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings

NWIPB OpenIR

	Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings
	Hu, Yan Feng ; Zhou, Guoying ; Na, Xiao Fan ; Yang, Lijing ; Nan, Wen Bin ; Liu, Xu ; Zhang, Yong Qiang ; Li, Jiao Long ; Bi, Yu Rong ; Bi, YR (reprint author), Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Gansu, Peoples R China.
	2013-07-15
发表期刊	JOURNAL OF PLANT PHYSIOLOGY ; Hu, YF; Zhou, GY; Na, XF; Yang, LJ; Nan, WB; Liu, X; Zhang, YQ; Li, JL; Bi, YR.Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings,JOURNAL OF PLANT PHYSIOLOGY,2013,170(11):965-975
摘要	Auxin and its homeostasis play key roles in many aspects of plant growth and development. Cadmium (Cd) is a phytotoxic heavy metal and its inhibitory effects on plant growth and development have been extensively studied. However, the underlying molecular mechanism of the effects of Cd stress on auxin homeostasis is still unclear. In the present study, we found that the root elongation, shoot weight, hypocotyl length and chlorophyll content in wild-type (WT) Arabidopsis seedlings were significantly reduced after exposure to Cd stress. However, the lateral root (LR) formation was markedly promoted by Cd stress. The level and distribution of auxin were both greatly altered in primary root tips and cotyledons of Cd-treated plants. The results also showed that after Cd treatment, the IAA content was significantly decreased, which was accompanied by increases in the activity of the IAA oxidase and alteration in the expression of several putative auxin biosynthetic and catabolic genes. Application of the auxin transport inhibitor, 1-naphthylphthalamic acid (NPA) and 1-naphthoxyacetic acid (1-NOA), reversed the effects of Cd on LR formation. Additionally, there was less promotion of LR formation by Cd treatment in aux1-7 and pin2 mutants than that in the WT. Meanwhile, Cd stress also altered the expression of PINs and AUX1 in Arabidopsis roots, implying that the auxin transport pathway is required for Cd-modulated LR development. Taken together, these findings suggest that Cd stress disturbs auxin homeostasis through affecting auxin level, distribution, metabolism, and transport in Arabidopsis seedling. (C) 2013 Elsevier GmbH. All rights reserved.; Auxin and its homeostasis play key roles in many aspects of plant growth and development. Cadmium (Cd) is a phytotoxic heavy metal and its inhibitory effects on plant growth and development have been extensively studied. However, the underlying molecular mechanism of the effects of Cd stress on auxin homeostasis is still unclear. In the present study, we found that the root elongation, shoot weight, hypocotyl length and chlorophyll content in wild-type (WT) Arabidopsis seedlings were significantly reduced after exposure to Cd stress. However, the lateral root (LR) formation was markedly promoted by Cd stress. The level and distribution of auxin were both greatly altered in primary root tips and cotyledons of Cd-treated plants. The results also showed that after Cd treatment, the IAA content was significantly decreased, which was accompanied by increases in the activity of the IAA oxidase and alteration in the expression of several putative auxin biosynthetic and catabolic genes. Application of the auxin transport inhibitor, 1-naphthylphthalamic acid (NPA) and 1-naphthoxyacetic acid (1-NOA), reversed the effects of Cd on LR formation. Additionally, there was less promotion of LR formation by Cd treatment in aux1-7 and pin2 mutants than that in the WT. Meanwhile, Cd stress also altered the expression of PINs and AUX1 in Arabidopsis roots, implying that the auxin transport pathway is required for Cd-modulated LR development. Taken together, these findings suggest that Cd stress disturbs auxin homeostasis through affecting auxin level, distribution, metabolism, and transport in Arabidopsis seedling. (C) 2013 Elsevier GmbH. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/31995
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Hu, Yan Feng,Zhou, Guoying,Na, Xiao Fan,et al. Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings[J]. JOURNAL OF PLANT PHYSIOLOGY, Hu, YF; Zhou, GY; Na, XF; Yang, LJ; Nan, WB; Liu, X; Zhang, YQ; Li, JL; Bi, YR.Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings,JOURNAL OF PLANT PHYSIOLOGY,2013,170(11):965-975,2013.
APA	Hu, Yan Feng.,Zhou, Guoying.,Na, Xiao Fan.,Yang, Lijing.,Nan, Wen Bin.,...&Bi, YR .(2013).Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings.JOURNAL OF PLANT PHYSIOLOGY.
MLA	Hu, Yan Feng,et al."Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings".JOURNAL OF PLANT PHYSIOLOGY (2013).