Inhibition of inducible nitric oxide synthase expression and nitric oxide production in plateau pika (Ochotona curzoniae) at high altitude on Qinghai-Tibet Plateau

NWIPB OpenIR

	Inhibition of inducible nitric oxide synthase expression and nitric oxide production in plateau pika (Ochotona curzoniae) at high altitude on Qinghai-Tibet Plateau
	Xie, Ling ; Zhang, Xuze ; Qi, Delin ; Guo, Xinyi ; Pang, Bo ; Du, Yurong ; Zou, Xiaoyan ; Guo, Songchang ; Zhao, Xinquan
	2014-04-30
发表期刊	NITRIC OXIDE-BIOLOGY AND CHEMISTRY ; Xie, L; Zhang, XZ; Qi, DL; Guo, XY; Pang, B; Du, YR; Zou, XY; Guo, SC; Zhao, XQ.Inhibition of inducible nitric oxide synthase expression and nitric oxide production in plateau pika (Ochotona curzoniae) at high altitude on Qinghai-Tibet Plateau,NITRIC OXIDE-BIOLOGY AND CHEMISTRY,2014,38():38
摘要	Nitric oxide (NO), a potent vasodilator, plays an important role in preventing hypoxia induced pulmonary hypertension. Endogenous NO is synthesized by nitric oxide synthases (NOSs) from L-arginine. In mammals, three different NOSs have been identified, including neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS). Plateau pika (Ochotona curzoniae) is a typical hypoxia tolerant mammal that lives at 3000-5000 m above sea level on the Qinghai-Tibet Plateau. The aim of this study was to investigate whether NOS expression and NO production are regulated by chronic hypoxia in plateau pika. Quantitative real-time PCR and western blot analyses were conducted to quantify relative abundances of iNOS and eNOS transcripts and proteins in the lung tissues of plateau pikas at different altitudes (4550, 3950 and 3200 m). Plasma NO metabolites, nitrite/nitrate (NOx-) levels were also examined by Ion chromatography to determine the correlation between NO production and altitude level. The results revealed that iNOS transcript levels were significantly lower in animals at high altitudes (decreased by 53% and 57% at altitude of 3950 and 4550 m compared with that at 3200 m). Similar trends in iNOS protein abundances were observed (26% and 41% at 3950 and 4550 m comparing with at 3200 m). There were no significant differences in eNOS mRNA and protein levels in the pika lungs among different altitudes. The plasma NO levels of the plateau pikas at high altitudes significantly decreased (1.65 +/- 0.19 mu g/mL at 3200 m to 0.44 +/- 0.03 mu g/mL at 3950 m and 0.24 +/- 0.01 mu g/mL at 4550 m). This is the first evidence describing the effects of chronic hypoxia on NOS expression and NO levels. in the plateau pika in high altitude adaptation. We conclude that iNOS expression and NO production are suppressed at high altitudes, and the lower NO concentration at high altitudes may serve crucial roles for helping the plateau pika to survive at hypoxic environment. (c) 2014 Elsevier Inc. All rights reserved.; Nitric oxide (NO), a potent vasodilator, plays an important role in preventing hypoxia induced pulmonary hypertension. Endogenous NO is synthesized by nitric oxide synthases (NOSs) from L-arginine. In mammals, three different NOSs have been identified, including neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS). Plateau pika (Ochotona curzoniae) is a typical hypoxia tolerant mammal that lives at 3000-5000 m above sea level on the Qinghai-Tibet Plateau. The aim of this study was to investigate whether NOS expression and NO production are regulated by chronic hypoxia in plateau pika. Quantitative real-time PCR and western blot analyses were conducted to quantify relative abundances of iNOS and eNOS transcripts and proteins in the lung tissues of plateau pikas at different altitudes (4550, 3950 and 3200 m). Plasma NO metabolites, nitrite/nitrate (NOx-) levels were also examined by Ion chromatography to determine the correlation between NO production and altitude level. The results revealed that iNOS transcript levels were significantly lower in animals at high altitudes (decreased by 53% and 57% at altitude of 3950 and 4550 m compared with that at 3200 m). Similar trends in iNOS protein abundances were observed (26% and 41% at 3950 and 4550 m comparing with at 3200 m). There were no significant differences in eNOS mRNA and protein levels in the pika lungs among different altitudes. The plasma NO levels of the plateau pikas at high altitudes significantly decreased (1.65 +/- 0.19 mu g/mL at 3200 m to 0.44 +/- 0.03 mu g/mL at 3950 m and 0.24 +/- 0.01 mu g/mL at 4550 m). This is the first evidence describing the effects of chronic hypoxia on NOS expression and NO levels. in the plateau pika in high altitude adaptation. We conclude that iNOS expression and NO production are suppressed at high altitudes, and the lower NO concentration at high altitudes may serve crucial roles for helping the plateau pika to survive at hypoxic environment. (c) 2014 Elsevier Inc. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/32295
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xie, Ling,Zhang, Xuze,Qi, Delin,et al. Inhibition of inducible nitric oxide synthase expression and nitric oxide production in plateau pika (Ochotona curzoniae) at high altitude on Qinghai-Tibet Plateau[J]. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, Xie, L; Zhang, XZ; Qi, DL; Guo, XY; Pang, B; Du, YR; Zou, XY; Guo, SC; Zhao, XQ.Inhibition of inducible nitric oxide synthase expression and nitric oxide production in plateau pika (Ochotona curzoniae) at high altitude on Qinghai-Tibet Plateau,NITRIC OXIDE-BIOLOGY AND CHEMISTRY,2014,38():38,2014.
APA	Xie, Ling.,Zhang, Xuze.,Qi, Delin.,Guo, Xinyi.,Pang, Bo.,...&Zhao, Xinquan.(2014).Inhibition of inducible nitric oxide synthase expression and nitric oxide production in plateau pika (Ochotona curzoniae) at high altitude on Qinghai-Tibet Plateau.NITRIC OXIDE-BIOLOGY AND CHEMISTRY.
MLA	Xie, Ling,et al."Inhibition of inducible nitric oxide synthase expression and nitric oxide production in plateau pika (Ochotona curzoniae) at high altitude on Qinghai-Tibet Plateau".NITRIC OXIDE-BIOLOGY AND CHEMISTRY (2014).