Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography

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	Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography
	He, Yanfeng ; Lv, Huanhuan ; Wang, Xiaoyan ; Suo, Yourui ; Wang, Honglun
	2014-01-31
发表期刊	SEPARATION SCIENCE AND TECHNOLOGY ; He, YF; Lv, HH; Wang, XY; Suo, YR; Wang, HL.Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography,SEPARATION SCIENCE AND TECHNOLOGY,2014,49(4):580
摘要	It is difficult to simultaneously isolate and purify flavonoids and polyhydroxystilbenes due to their similar polarities. In this study, we isolated and purified these compounds from Trigonella foenum-graecum L. seed extracts using high-speed counter-current chromatography (HSCCC) in two steps with two different kinds of solvent systems. In the first step, n-hexane:ethyl acetate:methanol:water (1:5:1:5, v/v) was used as the two-phase solvent system, desoxy-rhaponticin and rhapontigenin were purified, and orientin, vitexin, isovitexin, and rhaponticin were eluted together. In the second step, ethyl acetate:water (1:1, v/v) was used as the two-phase solvent system, and orientin, vitexin, rhaponticin, and isovitexin were isolated and purified. Purities of the isolated compounds were determined using HPLC, while UV spectra, H- 1 -NMR, and C- 13 -NMR aided in structure identification.; It is difficult to simultaneously isolate and purify flavonoids and polyhydroxystilbenes due to their similar polarities. In this study, we isolated and purified these compounds from Trigonella foenum-graecum L. seed extracts using high-speed counter-current chromatography (HSCCC) in two steps with two different kinds of solvent systems. In the first step, n-hexane:ethyl acetate:methanol:water (1:5:1:5, v/v) was used as the two-phase solvent system, desoxy-rhaponticin and rhapontigenin were purified, and orientin, vitexin, isovitexin, and rhaponticin were eluted together. In the second step, ethyl acetate:water (1:1, v/v) was used as the two-phase solvent system, and orientin, vitexin, rhaponticin, and isovitexin were isolated and purified. Purities of the isolated compounds were determined using HPLC, while UV spectra, H- 1 -NMR, and C- 13 -NMR aided in structure identification.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/32317
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	He, Yanfeng,Lv, Huanhuan,Wang, Xiaoyan,et al. Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY, He, YF; Lv, HH; Wang, XY; Suo, YR; Wang, HL.Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography,SEPARATION SCIENCE AND TECHNOLOGY,2014,49(4):580,2014.
APA	He, Yanfeng,Lv, Huanhuan,Wang, Xiaoyan,Suo, Yourui,&Wang, Honglun.(2014).Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography.SEPARATION SCIENCE AND TECHNOLOGY.
MLA	He, Yanfeng,et al."Isolation and Purification of Six Bioactive Compounds from the Seeds of Trigonella foenum-graecum L. using High-Speed Counter-Current Chromatography".SEPARATION SCIENCE AND TECHNOLOGY (2014).