Genetic variation in cultivated Rheum tanguticum populations

NWIPB OpenIR

	Genetic variation in cultivated Rheum tanguticum populations
	Hu, Yanping ; Xie, Xiaolong ; Wang, Li ; Zhang, Huaigang ; Yang, Jian ; Li, Yi
	2014
发表期刊	GENETICS AND MOLECULAR BIOLOGY ; Hu, YP; Xie, XL; Wang, L; Zhang, HG; Yang, J; Li, Y.Genetic variation in cultivated Rheum tanguticum populations,GENETICS AND MOLECULAR BIOLOGY,2014,37(3):540
摘要	To examine whether cultivation reduced genetic variation in the important Chinese medicinal plant Rheum tanguticum, the levels and distribution of genetic variation were investigated using ISSR markers. Fifty-eight R. tanguticum individuals from five cultivated populations were studied. Thirteen primers were used and a total of 320 DNA bands were scored. High levels of genetic diversity were detected in cultivated R. tanguticum (PPB = 82.19, H = 0.2498, H-B = 0.3231, I = 0.3812) and could be explained by the outcrossing system, as well as long-lived and human-mediated seed exchanges. Analysis of molecular variance (AMOVA) showed that more genetic variation was found within populations (76.1%) than among them (23.9%). This was supported by the coefficient of gene differentiation (G(st) = 0.2742) and Bayesian analysis (theta(B) = 0.1963). The Mantel test revealed no significant correlation between genetic and geographic distances among populations (r = 0.1176, p = 0.3686). UPGMA showed that the five cultivated populations were separated into three clusters, which was in good accordance with the results provided by the Bayesian software STRUCTURE (K = 3). A short domestication history and no artificial selection may be an effective way of maintaining and conserving the gene pools of wild R. tanguticum.; To examine whether cultivation reduced genetic variation in the important Chinese medicinal plant Rheum tanguticum, the levels and distribution of genetic variation were investigated using ISSR markers. Fifty-eight R. tanguticum individuals from five cultivated populations were studied. Thirteen primers were used and a total of 320 DNA bands were scored. High levels of genetic diversity were detected in cultivated R. tanguticum (PPB = 82.19, H = 0.2498, H-B = 0.3231, I = 0.3812) and could be explained by the outcrossing system, as well as long-lived and human-mediated seed exchanges. Analysis of molecular variance (AMOVA) showed that more genetic variation was found within populations (76.1%) than among them (23.9%). This was supported by the coefficient of gene differentiation (G(st) = 0.2742) and Bayesian analysis (theta(B) = 0.1963). The Mantel test revealed no significant correlation between genetic and geographic distances among populations (r = 0.1176, p = 0.3686). UPGMA showed that the five cultivated populations were separated into three clusters, which was in good accordance with the results provided by the Bayesian software STRUCTURE (K = 3). A short domestication history and no artificial selection may be an effective way of maintaining and conserving the gene pools of wild R. tanguticum.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/32323
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Hu, Yanping,Xie, Xiaolong,Wang, Li,et al. Genetic variation in cultivated Rheum tanguticum populations[J]. GENETICS AND MOLECULAR BIOLOGY, Hu, YP; Xie, XL; Wang, L; Zhang, HG; Yang, J; Li, Y.Genetic variation in cultivated Rheum tanguticum populations,GENETICS AND MOLECULAR BIOLOGY,2014,37(3):540,2014.
APA	Hu, Yanping,Xie, Xiaolong,Wang, Li,Zhang, Huaigang,Yang, Jian,&Li, Yi.(2014).Genetic variation in cultivated Rheum tanguticum populations.GENETICS AND MOLECULAR BIOLOGY.
MLA	Hu, Yanping,et al."Genetic variation in cultivated Rheum tanguticum populations".GENETICS AND MOLECULAR BIOLOGY (2014).