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Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization
Liu, Mengge ; Chen, Guang ; Guo, Hailong ; Fan, Baoki ; Liu, Jianjun ; Fu, Qiang ; Li, Xiu ; Lu, Xiaomin ; Zhao, Xianen ; Li, Guoliang ; Sun, Zhiwei ; Xia, Lian ; Zhu, Shuyun ; Yang, Daoshan ; Cao, Ziping ; Wang, Hua ; Suo, Yourui ; You, Jinmao
2015-09-16
发表期刊JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
摘要Determination of plant growth regulators (PGRs) in a signal transduction system (STS) is significant for transgenic food safety, but may be challenged by poor accuracy and analyte instability. In this work, a microwave-assisted extraction-derivatization (MAED) method is developed for six acidic PGRs in oil samples, allowing an efficient (<1.5 h) and facile (one step) pretreatment. Accuracies are greatly improved, particularly for gibberellin A(3) (-2.72 to -0.65%) as compared with those reported (-22 to -2%). Excellent selectivity and quite low detection limits (0.37-1.36 ng mL(-1)) are enabled by fluorescence detection-mass spectrum monitoring. Results show the significant differences in acidic PGRs between transgenic and nontransgenic oils, particularly 1-naphthaleneacetic acid (1-NAA), implying the PGRs induced variations of components and genes. This study provides, for the first time, an accurate and efficient determination for labile PGRs involved in STS and a promising concept for objectively evaluating the safety of transgenic foods.; Determination of plant growth regulators (PGRs) in a signal transduction system (STS) is significant for transgenic food safety, but may be challenged by poor accuracy and analyte instability. In this work, a microwave-assisted extraction-derivatization (MAED) method is developed for six acidic PGRs in oil samples, allowing an efficient (<1.5 h) and facile (one step) pretreatment. Accuracies are greatly improved, particularly for gibberellin A(3) (-2.72 to -0.65%) as compared with those reported (-22 to -2%). Excellent selectivity and quite low detection limits (0.37-1.36 ng mL(-1)) are enabled by fluorescence detection-mass spectrum monitoring. Results show the significant differences in acidic PGRs between transgenic and nontransgenic oils, particularly 1-naphthaleneacetic acid (1-NAA), implying the PGRs induced variations of components and genes. This study provides, for the first time, an accurate and efficient determination for labile PGRs involved in STS and a promising concept for objectively evaluating the safety of transgenic foods.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/32577
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Liu, Mengge,Chen, Guang,Guo, Hailong,et al. Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2015.
APA Liu, Mengge.,Chen, Guang.,Guo, Hailong.,Fan, Baoki.,Liu, Jianjun.,...&You, Jinmao.(2015).Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY.
MLA Liu, Mengge,et al."Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2015).
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