低氧对细胞免疫的作用及神经内分泌调节

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	低氧对细胞免疫的作用及神经内分泌调节
	白海波
学位类型	硕士
导师	杜继曾
	1993
学位授予单位	中国科学院西北高原生物研究所
学位授予地点	中国科学院西北高原生物研究所
学位专业	动物学
关键词	低氧 T－淋巴细胞转化神经内分泌调节
摘要	应激引起机体免疫功能变化，且受神经内分泌的调节。本研究以模拟高原低氧方法观察低氧应激作用于大鼠的细胞免疫功能以及神经内分泌节机制。结果表明：与对照组（2300m）相比，5000m和7000m低氧24小时大鼠的外周血淋巴细胞转化分别下降28％和41％（p＜0．05）；5000m低氧暴露时间为7天、20天时，大鼠外周血淋巴细胞转化分别下降34％和60％（p<0．01）。在摘除大鼠肾上腺后，5000m和7000m 24小时低氧仍得到与上述类似的结果，表明低氧抑制淋巴细胞转化与肾上腺皮质激素无关。同时，以高原土著动物高原鼠兔（Ochotona curzoniae）为对象则不表现这种低氧抑制的作用。表明具有良好的低氧耐受性。正常和肾上腺摘除大鼠第三脑室给予外源性CRF（Corticotropin - releasing factor ) (1μg/animal)表现与低氧作用类似的淋巴细胞转化下降现象。7000m小时低氧时第三脑室给予高效价（1:30,000）CRF抗血清，可以部分阻断低氧抑制作用。大鼠侧脑室注入5nM NE，淋巴细胞转化比对照下降29％（p＜0.01），7000m 10小时低氧暴露时，侧脑室注入α－受体阴断剂酚妥拉明(phentolamin)则使低氧抑制淋巴细胞转化从42％提高到21％（p<0.05）。RIA法检测外周血浆中CRF和皮质酮水平显示急性低氧大鼠血浆CRF、皮质酮水平均升高，高原鼠兔无明显变化。侧脑室注入NE，血浆CRF水平升高，与低氧作用类似，急性低氧注入酚妥拉明后则降低。因而，本研究提示：低氧可使细胞免疫受到抑制，CRF是介导急性低氧抑制细胞免疫的一种因子，而与肾上腺皮质激素的变化没有平行关系。NE的免疫调节可能通过CRF而发挥作用。高原鼠兔具有良好的低氧适应性。
其他摘要	Stress causes immune system change, and it was believed that the effect was regulated by neuroendocrine system. In this study, using simulated altitude, we examined the effect of hypoxia on cellular immune function and the mechanisms involved in the immune response to hypoxia stress in the rat and altitude native mammal animal (Ochotona curzoniae, Pika). The results show: Compared with control group (altitude of 2300 m), 5000 m and 7000 m acute hypoxia for 24h in intact rats resulted in 28% and 41% (p < 0.05) decrement in T-lymphocyte proliferation respectively. At altitude of 5000m, during 7 days and 20 days hypoxia, the reduction in lymphocyte proliferation was 34% and 60% (p < 0.01) respectively. Similar suppression of immune function in response to the acute hypoxia was found in adrenalectomized rats as well, but not found in Pika. In intact and adrenalectomized rats were treated by icv CRF (1 μg/animal), resulted in decrease in lymphocyte proliferation, similar to those showed in hypoxia stress. When both intact and adrenalectomized rats were pretreated by icv with CRF antiserum, the immunosuppressive effects of acute hypoxia were comparably blocked. Administration of NE (5 nmol, icv), compared with controls, T-lymphocyte proliferation decreased 29% (p < 0.01), treatment with injection phentolamin (α-receptor blocker) (icv), prior to 7000 m acute hypoxia exposure caused that hypoxia-induced suppression of lymphocyte proliferation attenuated from 42% to 21%. An immunomodulatory role for CRF is further supported by the findings that ir-CRF and corticosterone levels in peripheral plasma significiantly increased after acute hypoxia. None of those was observed in Pika. Similar plasma ir-CRF levels increase were noted after NE injection (icv). Administration of phentolamine before hypoxia, induced a decrease in plasma ir-CRF levels. These observations suggest that acute hypoxia suppress the cellular immune function, CRF plays a significant role in modulating the immune response to acute hypoxia, probably by adrenal-independent mechanisms. Suppressive effects of NE perhaps achieved through CRF. The plateau Pika has a exellent adaptation to hypoxia.
页数	47
语种	中文
文献类型	学位论文
条目标识符	http://210.75.249.4/handle/363003/3322
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	白海波. 低氧对细胞免疫的作用及神经内分泌调节[D]. 中国科学院西北高原生物研究所. 中国科学院西北高原生物研究所,1993.

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