Cleavage Information of Mono-Saccharide Derivatives with Electrospray Mass Spectrometry

NWIPB OpenIR

	Cleavage Information of Mono-Saccharide Derivatives with Electrospray Mass Spectrometry
	Sun Xue-Jun ; Sun Zhi-Wei ; Hu Bao-Jun ; Sheng Xiao ; You Jin-Mao
	2008-10-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
摘要	Studies on the MS cleavage information of mono-carbohydrate derivatives using the labeling reagent 1-(2-naphthyl) -3-methyl-5-pyrazolone (NMP) coupled with liquid chromatography/electrospray ionization mass spectrometry(LC-ESI-MS) are reported. Carbohydrate derivatives show sufficient ionization information under ESI-MS in positive-ion mode. An intense protonated molecular ion at m/z [M + H](+) and characteristic fragment ion at m/z 473.0 can be used for the accurately qualitative analysis of carbohydrates. No interferences for all aliphatic and aromatic aldehydes presented in natural environmental samples were observed clue to the highly specific parent mass-to-charge ratio and the characteristic fragment ions. The results from ESI-MS experiments, especially involving the characteristic fragment ions for the accurately qualitative analysis of carbohydrate derivatives, were emphasized. The carbohydrates from the hydrolyzed rape bee pollen sample including mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be identified. It will be possible to develop a promising ESI-MS technique for carbohydrate analysis. The developed method for the identification of carbohydrate compositions is very accurate and reliable.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/33232
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Sun Xue-Jun,Sun Zhi-Wei,Hu Bao-Jun,et al. Cleavage Information of Mono-Saccharide Derivatives with Electrospray Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008.
APA	Sun Xue-Jun,Sun Zhi-Wei,Hu Bao-Jun,Sheng Xiao,&You Jin-Mao.(2008).Cleavage Information of Mono-Saccharide Derivatives with Electrospray Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY.
MLA	Sun Xue-Jun,et al."Cleavage Information of Mono-Saccharide Derivatives with Electrospray Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (2008).