急性低氧及β-内啡肽对大鼠不同组织核酸表达调控的影响

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	急性低氧及β-内啡肽对大鼠不同组织核酸表达调控的影响
	王超云
学位类型	硕士
导师	杜继曾
	1998
学位授予单位	中国科学院西北高原生物研究所
学位授予地点	中国科学院西北高原生物研究所
学位专业	细胞及分子生理学
关键词	急性低氧核酸调控 Dna甲基化 Β-内啡肽纳曲酮 Dna含量 Rna含量大脑皮层肝心
摘要	应激影响了DNA的表达调控，从而使RNA的转录、合成发生改变，其中DNA甲基化参预了DNA的表达调控。本文利用RP-HPLC技术研究了急性低氧及β-EP对大鼠大脑皮层、肝、心组织基因表达调控的影响。结果表明：1）急性低氧（7km，24h）使大脑皮层RNA含量明显提高（P < 0.05）、DNA含量及甲基化水平降低；与低氧（7km，24h）组比较，大鼠脑室注入β-EP（0.02nmol,2ul）后进行低氧暴露（7km，24h）使大脑皮层RNA含量显著提高（P < 0.05）、DNA含量极度显著降低（P < 0.01）、DNA甲基化水平进一步下降；脑室注入β-EP受体阻断剂NTX（1mg/kg）后给予急性低氧（7km，24h）进一步提高了大脑皮层RNA含量（P < 0.05），DNA含量变化不显著其甲基化水平低于2.3km组，与低氧组相近。2）急性低氧（7km，24h）使心组织RNA和DNA的含量显著降低（P < 0.05）、DNA甲基化水平上升；与低氧（7km，24h）组比较，大鼠脑室注入β-EP（0.02nmol,2ul）后给予急性低氧（7km，24h）RNA的含量显著降低（P < 0.01）、DNA含量变化不显著、DNA甲基化水平有大幅度下降；脑室注入NTX（1mg/kg）后给予急性低氧（7km，24h）RNA的含量显著降低（P < 0.01）、DNA含量变化不显著、DNA甲基化水平高于2.3km组。3）急性低氧（7km，24h）使肝组织DNA含量显著上升（P < 0.01）、RNA含量变化不显著；与低氧（7km，24h）组比较，大鼠脑室注入β-EP（0.02nmol,2ul）后给予急性低氧RNA含量显著提高（P < 0.01）、DNA含量显著降低（P < 0.05）、DNA甲基化水平下降；脑室注入NTX（1mg/kg）后给予急性低氧（7km，24h）RNA含量显著高于对照（P < 0.01）、DNA含量与低氧组比较变化不大，DNA甲基化处于较高水平。综上所述，可结论如下：①急性低氧对各组织DNA的调控作用是不同的。造成DNA甲基化水平的改变，同时还引起DNA含量的改变：肝组织显著增高、心肌组织显著下降、大脑皮层变化不显著。②急性低氧下β-EP进一步降低了组织中DNA的甲基化水平，使RNA含量显著升高，但对DNA的合成具有抑制作用。
其他摘要	Stress influences DNA regulation, which results in the changes of RNA transcription. Among DNA regulation, DNA methylation plays a major role. Having researched the effect of Beta-endorphin on DNA regulation of cerebral cortex, liver and heart of rat in acute hypoxia by RP-HPLC, we observed: 1) acute hypoxia (7km, 24h) significantly increased RNA content in cerebral cortex (P < 0.05), but decreased DNA content and the level of DNA methylation; after intracerebroventricular administration of Beta-endorphin (0.02nmol, 2ul), RNA content in cerebral cortex rose obviously (P < 0.05) but DNA content was reduced significantly (P < 0.01), the level of DNA methylation decrease significantly; RNA content in cerebral cortex was enhanced significantly (P < 0.05) after icv Nultrexone (NTX 1mg/kg), DNA content went up noticeably comparing with hypoxia group. 2) acute hypoxia (7km, 24h) significantly decreased RNA and DNA content in heart (P < 0.05), the level of DNA methylation increased; after icv β-EP (0.02nmol, 2ul), RNA content in heart increased remarkably (P < 0.01), DNA content was never changed noticeably, the level of DNA methylation decrease; which can be reversed by icv Nultrexone. 3) acute hypoxia (7km, 24h) increased DNA content in liver very significantly (P < 0.01); after icv β-EP (0.02nmol 2ul), RNA content in liver elevated obviously (P < 0.01), but DNA content in liver and the level of DNA methylation declined significantly; Nultrexone alone caused RNA content in liver increasing significantly, DNA content in liver was never changed noticeably comparing hypoxia group, but the level of DNA methylation was higher. We draw a conclusion: ①The effect of acute hypoxia on DNA regulation of different organs in rat is different. Acute hypoxia changed the level of DNA methylation, at same time influenced DNA content as well as. It enhanced DNA content in liver and declined DNA content in heart, but never changed remarkably DNA content in cerebral cortex. ②β-EP further decreased the level of DNA methylation and cause RNA content increasing significantly, but inhabited DNA synthesis.
页数	37
语种	中文
文献类型	学位论文
条目标识符	http://210.75.249.4/handle/363003/3368
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	王超云. 急性低氧及β-内啡肽对大鼠不同组织核酸表达调控的影响[D]. 中国科学院西北高原生物研究所. 中国科学院西北高原生物研究所,1998.

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