红景天C02超临界萃取物的化学成分研究

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	红景天C02超临界萃取物的化学成分研究
	毕森
学位类型	硕士
导师	胡凤祖
	2010-05
学位授予单位	中国科学院研究生院
学位授予地点	北京
学位专业	植物学
关键词	红景天鞣质抗疲劳 Co2超临界萃取 Gc-ms
摘要	本论文对红景天CO2超临界萃取工艺进行了研究，并对其萃取物进行了系统分离和化合物的结构鉴定。并以提取过红景天苷和红景天多糖的残渣为原料，对鞣质的提取和纯化工艺进行了研究。取得了如下结果： 1. 红景天CO2超临界萃取的最适条件为：压力30 MPa；萃取温度50℃；二氧化碳流量为40 L/h；时间为2 h；萃取物的得率为1.813%。 2. 对红景天CO2超临界萃取物的化学成分进行GC-MS和HPLC分析，在萃取物中鉴定了21种化合物，其中石油醚部分鉴定了23种化合物，乙酸乙酯部分鉴定了12种化合物，正丁醇部分鉴定了3种化合物。 3. 对红景天CO2超临界萃取物的进行系统分离，得到5个化合物，分别是β-谷甾醇（β-stiosterol），3-甲氧基-4-羟基反式肉桂酸十二酯（n-dodecacosyl ferulate），酪醇（tyrosol），胡萝卜苷（dancosterol）和香草醛（vanillin）。 4. 红景天中总鞣质提取的最适条件为：丙酮浓度50%、料液比1：10、超声功率40 W、时间60 min，总鞣质的得率为11.84%。 5. 对红景天总鞣质纯化的工艺进行研究，得出最适条件为：用D101型大孔吸附树脂，先用水洗脱3 BV，以2 BV/h速度，50%乙醇洗脱3 BV，收集50%乙醇洗脱部位，所得鞣质含量在90 % 左右。 6. 对纯化前和纯化后的总鞣质进行鞣质含量的测定，结果显示纯化前鞣质含量为57.28%，纯化后的鞣质含量为89.55%。
其他摘要	In this paper, Chemical Constituents of Extract in Rhodiola Rosea L. by SFE-CO2 was studied and 5 compounds were identified.The process of tannin extraction and purification was studied，too.The results are as follows: 1. The optimal conditions of extract of Rhodiol by SFE-CO2 were: pressure 30 MPa; temperature 50 ℃; CO2 flow rate 40 L / h; time 3 h; the yield of extract was 1.813%. 2. To study the chemical composition of SFE-CO2 extract of Rhodiola by GC-MS and HPLC, 21 compounds were identified in the extracts, 23 compounds were identified in petroleum ether part, 12 compounds were identified in ethyl acetate part, 3 compounds were identified in n-butanol part. 3. To study the chemical composition of SFE-CO2 extract of Rhodiola, we separate it and get 5 compounds. they have been identified as follows： β- sitosterol, 3 - methoxy -4 -hydroxy-trans-cinnamic acid 12 ester, tyrosol, daucosterol, vanillin. 4. Extract total tannins of Rhodiola, the optimal conditions were: 50% acetone, solid to liquid ratio is 1:10, ultrasonic power 40 W, time 60 min, total tannin yield was 11.84 %. 5. The process of tannin purification was studied, the optimum conditions were: with D101 macroporous resin, washed with water 3 BV, the speed is 2 BV / h, 50% ethanol elution 3 BV, 50% ethanol elution parts was collected and its tannin contents was about 90%. 6. The tannin contents of before purification and after purification were tested,the results were the tannin content before purification were 57.28%, the tannin content after purification were 89.55%.
学科领域	生物科学
语种	中文
文献类型	学位论文
条目标识符	http://210.75.249.4/handle/363003/3387
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	毕森. 红景天C02超临界萃取物的化学成分研究[D]. 北京. 中国科学院研究生院,2010.

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