Knowledge Management System of Northwest Institute of Plateau Biology, CAS
A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles | |
Qu, Fei ; Huang, Wei ; You, Jinmao | |
2018-02-01 | |
发表期刊 | COLLOIDS AND SURFACES B-BIOINTERFACES |
摘要 | In this work, we report a fluorescence strategy for detecting dopamine (DA) and sensing tyrosinase (TYR) activity on the basis of the dual-emission carbon dots (DECDs), which contain two emitters: the blue emitters (BE, maximum emission at 385 nm) and yellow emitters (YE, maximum emission at 530 nm). Gold nanoparticles (AuNPs) can effectively quench the two emissions of DECDs. The addition of DA aggregates AuNPs effectively, leading to the fluorescence recovery of dual emitters gradually. This strategy exhibits a high selectivity toward DA and shows good linear ranges, such as 0.5-3 mu M for BE and 0.1-3 mu M for YE. Additionally, the proposed method is successfully applied to the determination of DA in real samples with satisfactory recoveries. Subsequently, this DECDs-AuNPs platform is further taken advantage to assess TYR activity by the aid of TYR's capability for oxidation of DA into dopaquinone, which will not induce the agglomeration of AuNPs, so the fluorescence quenching of DECDs is associated with TYR activity. Finally, the mechanism of the reaction is discussed in detail, and the results suggest that both amine and phenolic hydroxyl groups of DA bring the aggregation of AuNPs. (C) 2017 Elsevier B.V. All rights reserved. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/34006 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Qu, Fei,Huang, Wei,You, Jinmao. A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2018. |
APA | Qu, Fei,Huang, Wei,&You, Jinmao.(2018).A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles.COLLOIDS AND SURFACES B-BIOINTERFACES. |
MLA | Qu, Fei,et al."A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles".COLLOIDS AND SURFACES B-BIOINTERFACES (2018). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论