Cytological and molecular identification of alien chromatin in giant spike wheat germplasm

NWIPB OpenIR

	Cytological and molecular identification of alien chromatin in giant spike wheat germplasm
	Dou, QW ; Chen, PD ; Xie, JF
	2003-09-01
发表期刊	ACTA BOTANICA SINICA ; Dou Quanwen,Chen Peidu,Xie Junfeng.Cytological and Molecular Identification of Alien Chromatin in Giant Spike Wheat Germplasm.Acta Botanica Sinica,2003,45(9):1109-1115
摘要	Alien chromosomes of twelve giant spike wheat germplasm lines were identified by C-banding, genomic in situ hybridization (GISH), sequence characterized amplified region (SCAR), and random amplified polymorphic DNA (RAPD). All lines showed a chromosome number of 2n = 42, five of them carried both a pair of wheat-rye (Triticum aestivum-Secale cereal) 1BL/1RS translocation chromosomes and a pair of Agropyron intermedium (Ai) chromosomes, three carried a pair of Ai chromosomes only, three others carried a pair of 1BL/1RS chromosomes only, and one carried neither 1BL/1BS nor Ai chromosome. Further identification revealed that the identical Ai chromosome in these germplasm lines substituted the chromosome 2D of common wheat (Triticum aestivum L.), designated as 2Ai. The genetic implication and further utilization of 2Ai in wheat improvement were also discussed.; Alien chromosomes of twelve giant spike wheat germplasm lines were identified by C-banding, genomic in situ hybridization (GISH), sequence characterized amplified region (SCAR), and random amplified polymorphic DNA (RAPD). All lines showed a chromosome number of 2n = 42, five of them carried both a pair of wheat-rye (Triticum aestivum-Secale cereal) 1BL/1RS translocation chromosomes and a pair of Agropyron intermedium (Ai) chromosomes, three carried a pair of Ai chromosomes only, three others carried a pair of 1BL/1RS chromosomes only, and one carried neither 1BL/1BS nor Ai chromosome. Further identification revealed that the identical Ai chromosome in these germplasm lines substituted the chromosome 2D of common wheat (T aestivum L.), designated as 2Ai. The genetic implication and further utilization of 2Ai in wheat improvement were also discussed.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/34950
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Dou, QW,Chen, PD,Xie, JF. Cytological and molecular identification of alien chromatin in giant spike wheat germplasm[J]. ACTA BOTANICA SINICA, Dou Quanwen,Chen Peidu,Xie Junfeng.Cytological and Molecular Identification of Alien Chromatin in Giant Spike Wheat Germplasm.Acta Botanica Sinica,2003,45(9):1109-1115,2003.
APA	Dou, QW,Chen, PD,&Xie, JF.(2003).Cytological and molecular identification of alien chromatin in giant spike wheat germplasm.ACTA BOTANICA SINICA.
MLA	Dou, QW,et al."Cytological and molecular identification of alien chromatin in giant spike wheat germplasm".ACTA BOTANICA SINICA (2003).