Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae)

NWIPB OpenIR

	Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae)
	Wang, Ailan ; Zhang, Qian ; Wan, Dongshi ; Yang, Yanzhuo ; Liu, Jianquan
	2008
发表期刊	CONSERVATION GENETICS ; Wang Ailan,Zhang Qian,Wan Dongshi,Yang Yanzhuo,Liu Jianquan.Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae).CONSERVATION GENETICS,2008,9(4):969-971
摘要	Hippophae rhamnoides ssp. sinensis occurs mainly in the and regions of northwest China. The wood stands of this subspecies play an important role in maintaining the local ecosystems in these regions. In addition, the genetic characteristics are essential to understand the historical range changes of this subspecies and its morphological differentiation with other subspecies. In this study, we developed nine microsatellite loci for this subspecies for the first time. We used the combining biotin capture method to enrich AG/CT/AC/GT/CG/GTG/CCA microsatellites. Twenty-six microsatellites were isolated from the enriching library and nine of them were found to be polymorphic through screening 12 distantly distributed individuals. The number of alleles per locus ranged from three to twelve and expected heterozygosity from 0.2659 to 0.4767, respectively. We further performed cross-priming tests in another subspecies and two congeneric species. These firstly isolated loci will provide a useful tool to investigate the genetic structure of this subspecies and its morphological differentiation from the other subspecies.; Hippophae rhamnoides ssp. sinensis occurs mainly in the and regions of northwest China. The wood stands of this subspecies play an important role in maintaining the local ecosystems in these regions. In addition, the genetic characteristics are essential to understand the historical range changes of this subspecies and its morphological differentiation with other subspecies. In this study, we developed nine microsatellite loci for this subspecies for the first time. We used the combining biotin capture method to enrich AG/CT/AC/GT/CG/GTG/CCA microsatellites. Twenty-six microsatellites were isolated from the enriching library and nine of them were found to be polymorphic through screening 12 distantly distributed individuals. The number of alleles per locus ranged from three to twelve and expected heterozygosity from 0.2659 to 0.4767, respectively. We further performed cross-priming tests in another subspecies and two congeneric species. These firstly isolated loci will provide a useful tool to investigate the genetic structure of this subspecies and its morphological differentiation from the other subspecies.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/35427
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Ailan,Zhang, Qian,Wan, Dongshi,et al. Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae)[J]. CONSERVATION GENETICS, Wang Ailan,Zhang Qian,Wan Dongshi,Yang Yanzhuo,Liu Jianquan.Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae).CONSERVATION GENETICS,2008,9(4):969-971,2008.
APA	Wang, Ailan,Zhang, Qian,Wan, Dongshi,Yang, Yanzhuo,&Liu, Jianquan.(2008).Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae).CONSERVATION GENETICS.
MLA	Wang, Ailan,et al."Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae)".CONSERVATION GENETICS (2008).