ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae)

NWIPB OpenIR

	ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae)
	Xia, Tao ; Chen, Shilong ; Chen, Shenyun ; Zhang, Defang ; Zhang, Dejun ; Gao, Qingbo ; Ge, Xuejun
	2007-04-01
发表期刊	BIOCHEMICAL SYSTEMATICS AND ECOLOGY ; Xia Tao, Chen Shilong,Chen Shenyun,Zhang Defang,Zhang Dejun,Gao Qingbo,Ge, Xuejun.ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae).BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2007,35(4):209-214
摘要	Inter-simple sequence repeat markers (ISSR) were used to estimate genetic diversity within and among 10 populations of Rhodiola chrysanthemifolia along Nianqingtangula Mountains and Brahmaputra, a species endemic to the Qinghai-Tibet Plateau and an endangered medicinal plant. Of the 100 primers screened, 13 produced highly polymorphic DNA fragments. Using these primers, 116 discernible DNA fragments were generated of which 104 (89.7%) were polymorphic, indicating substantial genetic diversity at the species level. Genetic diversity measured by the percentage of polymorphic bands (PPB) at the population level ranged from 21.97% to 48.8%. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly among populations (77.3%), but no regional differentiation was discernible. Variance within populations was only 22.7%. The main factor responsible for this high level of differentiation among populations is probably the historical geographical and genetic isolation of populations in a harsh mountainous environment. Concerning the management of R. chrysanthemifolia, the high genetic differentiation of populations indicates the necessity of conserving the maximum possible number of populations. (c) 2006 Elsevier Ltd. All rights reserved.; Inter-simple sequence repeat markers (ISSR) were used to estimate genetic diversity within and among 10 populations of Rhodiola chrysanthemifolia along Nianqingtangula Mountains and Brahmaputra, a species endemic to the Qinghai-Tibet Plateau and an endangered medicinal plant. Of the 100 primers screened, 13 produced highly polymorphic DNA fragments. Using these primers, 116 discernible DNA fragments were generated of which 104 (89.7%) were polymorphic, indicating substantial genetic diversity at the species level. Genetic diversity measured by the percentage of polymorphic bands (PPB) at the population level ranged from 21.97% to 48.8%. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly among populations (77.3%), but no regional differentiation was discernible. Variance within populations was only 22.7%. The main factor responsible for this high level of differentiation among populations is probably the historical geographical and genetic isolation of populations in a harsh mountainous environment. Concerning the management of R. chrysanthemifolia, the high genetic differentiation of populations indicates the necessity of conserving the maximum possible number of populations. (c) 2006 Elsevier Ltd. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/35504
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xia, Tao,Chen, Shilong,Chen, Shenyun,et al. ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae)[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, Xia Tao, Chen Shilong,Chen Shenyun,Zhang Defang,Zhang Dejun,Gao Qingbo,Ge, Xuejun.ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae).BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2007,35(4):209-214,2007.
APA	Xia, Tao.,Chen, Shilong.,Chen, Shenyun.,Zhang, Defang.,Zhang, Dejun.,...&Ge, Xuejun.(2007).ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae).BIOCHEMICAL SYSTEMATICS AND ECOLOGY.
MLA	Xia, Tao,et al."ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae)".BIOCHEMICAL SYSTEMATICS AND ECOLOGY (2007).