Molecular characterization of LMW glutenin genes from Taeniatherum Nevski

NWIPB OpenIR

	Molecular characterization of LMW glutenin genes from Taeniatherum Nevski
	Han, Chang ; Yan, Ze-Hong ; Dai, Shou-Fen ; Liu, Deng-Cai ; Wei, Yu-Ming ; Zheng, You-Liang ; Lan, Xiu-Jin ; Peng, Yuan-Ying
	2011-10-01
发表期刊	GENETIC RESOURCES AND CROP EVOLUTION ; Han, C; Yan, ZH; Dai, SF; Liu, DC; Wei, YM; Zheng, YL; Lan, XJ; Peng, YY.Molecular characterization of LMW glutenin genes from Taeniatherum Nevski,GENETIC RESOURCES AND CROP EVOLUTION,2011,58(7):1029-1039
摘要	We characterized 45 LMW glutenin genes from three diploid species of Taeniatherum using 63 primer combinations, designed according to 264 genes reported in wheat and related species. The genes had 909-1,059 bp nucleotides and 301-351 amino acids. The deduced peptides shared similar structures with LMW-m proteins of wheat. The 45 genes shared 77.2-99.7% identities in peptide sequence among each other and 60.0-82.0% identities to proteins from wheat and related species. They were divided into five types according to the N-terminals, starting with METSCIP-, METSRVP-, METGRIP-, METGSIP- and VETSCIP-. The last three and some other structural domain variations were not reported previously in the Triticeae. Thirty-three genes encoded full mature proteins with intact ORFs, whereas the other 12 were pseudogenes with incomplete ORFs, in-frame stop codons or frame-shift mutations. Phylogenetic analysis showed that orthologous genes from Taeniatherum were more similar to those in the B and D genomes than in the A genome.; We characterized 45 LMW glutenin genes from three diploid species of Taeniatherum using 63 primer combinations, designed according to 264 genes reported in wheat and related species. The genes had 909-1,059 bp nucleotides and 301-351 amino acids. The deduced peptides shared similar structures with LMW-m proteins of wheat. The 45 genes shared 77.2-99.7% identities in peptide sequence among each other and 60.0-82.0% identities to proteins from wheat and related species. They were divided into five types according to the N-terminals, starting with METSCIP-, METSRVP-, METGRIP-, METGSIP- and VETSCIP-. The last three and some other structural domain variations were not reported previously in the Triticeae. Thirty-three genes encoded full mature proteins with intact ORFs, whereas the other 12 were pseudogenes with incomplete ORFs, in-frame stop codons or frame-shift mutations. Phylogenetic analysis showed that orthologous genes from Taeniatherum were more similar to those in the B and D genomes than in the A genome.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/35717
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Han, Chang,Yan, Ze-Hong,Dai, Shou-Fen,et al. Molecular characterization of LMW glutenin genes from Taeniatherum Nevski[J]. GENETIC RESOURCES AND CROP EVOLUTION, Han, C; Yan, ZH; Dai, SF; Liu, DC; Wei, YM; Zheng, YL; Lan, XJ; Peng, YY.Molecular characterization of LMW glutenin genes from Taeniatherum Nevski,GENETIC RESOURCES AND CROP EVOLUTION,2011,58(7):1029-1039,2011.
APA	Han, Chang.,Yan, Ze-Hong.,Dai, Shou-Fen.,Liu, Deng-Cai.,Wei, Yu-Ming.,...&Peng, Yuan-Ying.(2011).Molecular characterization of LMW glutenin genes from Taeniatherum Nevski.GENETIC RESOURCES AND CROP EVOLUTION.
MLA	Han, Chang,et al."Molecular characterization of LMW glutenin genes from Taeniatherum Nevski".GENETIC RESOURCES AND CROP EVOLUTION (2011).