A simple method for isolation and structural identification of arctigenin from Saussurea medusa Maxim. by preparation chromatography and single crystal X-ray diffraction

NWIPB OpenIR

	A simple method for isolation and structural identification of arctigenin from Saussurea medusa Maxim. by preparation chromatography and single crystal X-ray diffraction
	Yu, RT ; Liu, Z ; Yu, RX ; Zhang, HG ; Shao, Y ; Mei, LJ ; Tao, YD
	2011
发表期刊	JOURNAL OF MEDICINAL PLANTS RESEARCH ; Yu, RT; Liu, Z; Yu, RX; Zhang, HG; Shao, Y; Mei, LJ; Tao, YD.A simple method for isolation and structural identification of arctigenin from Saussurea medusa Maxim. by preparation chromatography and single crystal X-ray diffraction,JOURNAL OF MEDICINAL PLANTS RESEARCH,2011,5(6):979-983
摘要	Arctigenin was successfully isolated and purified from the crude extract of Saussurea medusa Maxim. by automatic flash preparation chromatography (AFPC). Chromatographic separation was performed with a gradient elution of (A) petroleum ether and (B) acetone. The qualitative analysis was accomplished with thin layer chromatography (TLC) under the following conditions: petroleum ether-acetone (5:3, v/v) was used as a developing agent and improved Dragendorff's reagent as a coloring agent at 105 degrees C. The rate of flow is about 0.38, the spot showed reddish yellow. The purity of arctigenin is 99.371%, which was determined by Agilent 1200 high performance liquid chromatograph (HPLC). Its structure was identified by single crystal X-ray diffraction. Arctigenin crystallized in the orthorhombic system, space group P2(1)/n; The unit cell dimensions are as follows: a=9.5649(11) angstrom, b=10.0929(12) angstrom, c=20.133(2) angstrom, alpha=90 degrees, beta=90 degrees, gamma=90 degrees, Z=4.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/35751
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yu, RT,Liu, Z,Yu, RX,et al. A simple method for isolation and structural identification of arctigenin from Saussurea medusa Maxim. by preparation chromatography and single crystal X-ray diffraction[J]. JOURNAL OF MEDICINAL PLANTS RESEARCH, Yu, RT; Liu, Z; Yu, RX; Zhang, HG; Shao, Y; Mei, LJ; Tao, YD.A simple method for isolation and structural identification of arctigenin from Saussurea medusa Maxim. by preparation chromatography and single crystal X-ray diffraction,JOURNAL OF MEDICINAL PLANTS RESEARCH,2011,5(6):979-983,2011.
APA	Yu, RT.,Liu, Z.,Yu, RX.,Zhang, HG.,Shao, Y.,...&Tao, YD.(2011).A simple method for isolation and structural identification of arctigenin from Saussurea medusa Maxim. by preparation chromatography and single crystal X-ray diffraction.JOURNAL OF MEDICINAL PLANTS RESEARCH.
MLA	Yu, RT,et al."A simple method for isolation and structural identification of arctigenin from Saussurea medusa Maxim. by preparation chromatography and single crystal X-ray diffraction".JOURNAL OF MEDICINAL PLANTS RESEARCH (2011).