Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identification

NWIPB OpenIR

	Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identification
	Suo, Yourui ; Wang, Lingyun
	2010-03-01
发表期刊	EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY ; Suo, YR; Wang, LY.Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identification,EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,2010,112(3):390-399
摘要	The seed lipid of Nitraria tangutorum was extracted by supercritical carbon dioxide extraction, microwave-assisted reflux extraction, ultrasound-assisted extraction, or solvent reflux extraction. The experimental parameters of supercritical carbon dioxide extraction including pressure, temperature, particle size, and extraction time were investigated. A facile and sensitive method for the simultaneous determination of 30 saturated and 9 unsaturated fatty acids by HPLC with fluorescence detection after pre-column derivatization was developed. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C(8) column in conjunction with gradient elution. Identification of fatty acid derivatives was carried out by on-line APCI/MS in positive-ion mode. Excellent quantitative linear responses of the 39 fatty acids were observed in the range of 0.014 to 14 mu mol/L with correlation coefficients higher than 0.9992. Limits of detection were in the range of 0.32-3.7 nmol/L (S/N = 3). The fatty acids in Nitraria tangutorum seed lipid with or without saponification extracted by the four different methods were determined and compared. The results indicated that the mass percentage of unsaturated fatty acids (mainly oleic acid, linoleic acid and linolenic acid) in Nitraria tangutorum seed lipid was up to 79%, and the best method was supercritical carbon dioxide extraction.; The seed lipid of Nitraria tangutorum was extracted by supercritical carbon dioxide extraction, microwave-assisted reflux extraction, ultrasound-assisted extraction, or solvent reflux extraction. The experimental parameters of supercritical carbon dioxide extraction including pressure, temperature, particle size, and extraction time were investigated. A facile and sensitive method for the simultaneous determination of 30 saturated and 9 unsaturated fatty acids by HPLC with fluorescence detection after pre-column derivatization was developed. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C(8) column in conjunction with gradient elution. Identification of fatty acid derivatives was carried out by on-line APCI/MS in positive-ion mode. Excellent quantitative linear responses of the 39 fatty acids were observed in the range of 0.014 to 14 mu mol/L with correlation coefficients higher than 0.9992. Limits of detection were in the range of 0.32-3.7 nmol/L (S/N = 3). The fatty acids in Nitraria tangutorum seed lipid with or without saponification extracted by the four different methods were determined and compared. The results indicated that the mass percentage of unsaturated fatty acids (mainly oleic acid, linoleic acid and linolenic acid) in Nitraria tangutorum seed lipid was up to 79%, and the best method was supercritical carbon dioxide extraction.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/35834
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Suo, Yourui,Wang, Lingyun. Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identification[J]. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, Suo, YR; Wang, LY.Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identification,EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,2010,112(3):390-399,2010.
APA	Suo, Yourui,&Wang, Lingyun.(2010).Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identification.EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY.
MLA	Suo, Yourui,et al."Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identification".EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY (2010).