Dual-Sensitive Probe of 2-(Benzoacridin) ethyl-imidazole-1-carboxylate for Determination of Aliphatic Amine with Fluorescence and Online Atmospheric Pressure Chemical Ionization Mass Spectrometry Identification

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	Dual-Sensitive Probe of 2-(Benzoacridin) ethyl-imidazole-1-carboxylate for Determination of Aliphatic Amine with Fluorescence and Online Atmospheric Pressure Chemical Ionization Mass Spectrometry Identification
	Fu Yan-Yan ; Li Xiao-Yan ; Sun Zhi-Wei ; Qin Xue-Qin ; Xia Lian ; Suo You-Rui ; Li Yu-Lin ; You Jin-Mao
	2010
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ; Fu, YY; Li, XY; Sun, ZW; Qin, XQ; Xia, L; Suo, YR; Li, YL; You, JM.Dual-Sensitive Probe of 2-(Benzoacridin) ethyl-imidazole-1-carboxylate for Determination of Aliphatic Amine with Fluorescence and Online Atmospheric Pressure Chemical Ionization Mass Spectrometry Identification,CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2010,38(1):8-14
摘要	With comparison of three different methods for the marking of amines compound, an optimal derivatization method was selected. 5-(2-Hydroxyethyl) benzoacridine (HBA) reacts with coupling agent N, N'-carbonyldiimidazole (CDI) to form an activated amide intermediate 2-(benzoacridin) ethyl-imidazole-1-carboxylate(BAEIC). BAEIC, which is dual-sensitive probe, reacts preferably with amino compounds at 80 degrees C in the presence of 4-dimethylaminopyridine (DMAP) catalyst in N, N-dimethylformamide (DMF) solvent to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda(ex) of 280 nm and an emission maximum at lambda(em) of 510 nm. BAEIC-amine derivatives simultaneously exhibited high. ionization potential with percent ionization (changing from 5.62% to 58.08% in aqueous acetonitrile and from 2.14% to 56.58% in aqueous methanol. Derivatives were not only sensitive to fluorescence but also to MS ionizable potential. The fluorescence detection limits(S/N = 3) were 0.12 - 0. 59 mu g/L. The online APCI-MS detection limits were 1.9-14 mu g/L (S/N = 5); With comparison of three different methods for the marking of amines compound, an optimal derivatization method was selected. 5-(2-Hydroxyethyl) benzoacridine (HBA) reacts with coupling agent N, N'-carbonyldiimidazole (CDI) to form an activated amide intermediate 2-(benzoacridin) ethyl-imidazole-1-carboxylate(BAEIC). BAEIC, which is dual-sensitive probe, reacts preferably with amino compounds at 80 degrees C in the presence of 4-dimethylaminopyridine (DMAP) catalyst in N, N-dimethylformamide (DMF) solvent to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda(ex) of 280 nm and an emission maximum at lambda(em) of 510 nm. BAEIC-amine derivatives simultaneously exhibited high. ionization potential with percent ionization (changing from 5.62% to 58.08% in aqueous acetonitrile and from 2.14% to 56.58% in aqueous methanol. Derivatives were not only sensitive to fluorescence but also to MS ionizable potential. The fluorescence detection limits(S/N = 3) were 0.12 - 0. 59 mu g/L. The online APCI-MS detection limits were 1.9-14 mu g/L (S/N = 5)
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/35850
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Fu Yan-Yan,Li Xiao-Yan,Sun Zhi-Wei,et al. Dual-Sensitive Probe of 2-(Benzoacridin) ethyl-imidazole-1-carboxylate for Determination of Aliphatic Amine with Fluorescence and Online Atmospheric Pressure Chemical Ionization Mass Spectrometry Identification[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, Fu, YY; Li, XY; Sun, ZW; Qin, XQ; Xia, L; Suo, YR; Li, YL; You, JM.Dual-Sensitive Probe of 2-(Benzoacridin) ethyl-imidazole-1-carboxylate for Determination of Aliphatic Amine with Fluorescence and Online Atmospheric Pressure Chemical Ionization Mass Spectrometry Identification,CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2010,38(1):8-14,2010.
APA	Fu Yan-Yan.,Li Xiao-Yan.,Sun Zhi-Wei.,Qin Xue-Qin.,Xia Lian.,...&You Jin-Mao.(2010).Dual-Sensitive Probe of 2-(Benzoacridin) ethyl-imidazole-1-carboxylate for Determination of Aliphatic Amine with Fluorescence and Online Atmospheric Pressure Chemical Ionization Mass Spectrometry Identification.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY.
MLA	Fu Yan-Yan,et al."Dual-Sensitive Probe of 2-(Benzoacridin) ethyl-imidazole-1-carboxylate for Determination of Aliphatic Amine with Fluorescence and Online Atmospheric Pressure Chemical Ionization Mass Spectrometry Identification".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (2010).