NWIPB OpenIR
Improved Method for the Extraction and Determination of Bromophenols in Seafoods by High-Performance Liquid Chromatography with Fluorescence Detection
Zhang, Shijuan1,3; Li, Yanxin2; You, Jinmao1,2; Wang, Hua2; Zheng, Yan4; Suo, Yourui1
2012-11-07
发表期刊JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN0021-8561
卷号60期号:44页码:10985-10990
文章类型Article
摘要A sensitive precolumn derivatization method using 10-methylacridone-2-sulfonyl chloride (MASC) as derivatizing reagent followed by high-performance liquid chromatography (HPLC) with fluorescence detection has been developed for the determination of naturally occurring bromophenols in seafoods. Instead of using a traditional complex steam distillation solvent extraction method, the extraction method was modified by using sulfuric acid hydrolysis followed by hexane extraction and subsequent alkaline back extraction to obtain good recoveries and fewer interferences. Batch analysis of bromophenols thus became more performable because the sample amount and analysis time were greatly reduced. The recoveries of the five bromophenols were >80% with a noticeable improvement for 4-BP, the recoveries of which were usually <40% when analyzed by traditional methods. The HPLC sensitivity for the determination of bromophenols was greatly enhanced through derivatization. Under optimal conditions, the quantification limits obtained by using 4.0 g of sample were in the range of 0.60-1.0 ng/g. This is the first time that derivatization was applied to enhance the HPLC sensitivity of bromophenols. The proposed method was successfully applied to the determination of the target compounds in seafoods with a much higher sensitivity than traditional HPLC method.; A sensitive precolumn derivatization method using 10-methylacridone-2-sulfonyl chloride (MASC) as derivatizing reagent followed by high-performance liquid chromatography (HPLC) with fluorescence detection has been developed for the determination of naturally occurring bromophenols in seafoods. Instead of using a traditional complex steam distillation solvent extraction method, the extraction method was modified by using sulfuric acid hydrolysis followed by hexane extraction and subsequent alkaline back extraction to obtain good recoveries and fewer interferences. Batch analysis of bromophenols thus became more performable because the sample amount and analysis time were greatly reduced. The recoveries of the five bromophenols were >80% with a noticeable improvement for 4-BP, the recoveries of which were usually <40% when analyzed by traditional methods. The HPLC sensitivity for the determination of bromophenols was greatly enhanced through derivatization. Under optimal conditions, the quantification limits obtained by using 4.0 g of sample were in the range of 0.60-1.0 ng/g. This is the first time that derivatization was applied to enhance the HPLC sensitivity of bromophenols. The proposed method was successfully applied to the determination of the target compounds in seafoods with a much higher sensitivity than traditional HPLC method.
关键词Bromophenols Hplc Fluorescence Seafood
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]MASS SPECTROMETRY ; ENHANCEMENT ; PRAWNS
收录类别SCI ; IC
语种英语
WOS研究方向Agriculture ; Chemistry ; Food Science & Technology
WOS类目Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS记录号WOS:000311192200014
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/3586
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China
2.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Qufu, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Yantai Vocat Coll, Art Dept Design, Yantai, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Shijuan,Li, Yanxin,You, Jinmao,et al. Improved Method for the Extraction and Determination of Bromophenols in Seafoods by High-Performance Liquid Chromatography with Fluorescence Detection[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2012,60(44):10985-10990.
APA Zhang, Shijuan,Li, Yanxin,You, Jinmao,Wang, Hua,Zheng, Yan,&Suo, Yourui.(2012).Improved Method for the Extraction and Determination of Bromophenols in Seafoods by High-Performance Liquid Chromatography with Fluorescence Detection.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,60(44),10985-10990.
MLA Zhang, Shijuan,et al."Improved Method for the Extraction and Determination of Bromophenols in Seafoods by High-Performance Liquid Chromatography with Fluorescence Detection".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 60.44(2012):10985-10990.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Improved Method for (469KB) 开放获取--浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Shijuan]的文章
[Li, Yanxin]的文章
[You, Jinmao]的文章
百度学术
百度学术中相似的文章
[Zhang, Shijuan]的文章
[Li, Yanxin]的文章
[You, Jinmao]的文章
必应学术
必应学术中相似的文章
[Zhang, Shijuan]的文章
[Li, Yanxin]的文章
[You, Jinmao]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Improved Method for the Extraction and Determination of Bromophenols in Seafoods by High-Performance Liquid.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。