Preparation of a Novel Glucuronomannan from Auricularia auricala and its Immunological Activity

NWIPB OpenIR

	Preparation of a Novel Glucuronomannan from Auricularia auricala and its Immunological Activity
	Li, Zonghong 1; Jiang, Dan 1; Bi, Hongtao 2; Liu, Dazheng 1; Jang, Sungju ; Zhou, Yifa 1
	2012-11-01
发表期刊	NATURAL PRODUCT COMMUNICATIONS
ISSN	1934-578X
卷号	7 期号:11 页码:1507-1510
文章类型	Article
摘要	A glucuronomannan (AA-4-H, Mw around 4 KDa) was prepared from the fruit bodies of Auricularia auricala by extraction with hot water, deproteination by Sevag reagent, stepwise precipitation with ethanol and partial acid hydrolysis. Monosaccharides analysis revealed that AA-4-H consisted of 91% mannose (Man) and 9% glucuronic acid (GlcA). FT-IR, NMR and methylation analyses indicated that AA-4-H is a branched glucuronomannan. Its main chains are composed of 1, 3-linked alpha-Manp, side chains are single alpha-Manp or alpha-GlcA residues attached to the O-2 and O-6 of Man residues of the main chains. Bioassay indicated that AA-4-H remarkably enhanced B lymphocyte proliferation and increased the production of nitric oxide of macrophages in vitro. Thus, glucuronomannan AA-4-H could be explored as a potential immunostimulation agent.; A glucuronomannan (AA-4-H, Mw around 4 KDa) was prepared from the fruit bodies of Auricularia auricala by extraction with hot water, deproteination by Sevag reagent, stepwise precipitation with ethanol and partial acid hydrolysis. Monosaccharides analysis revealed that AA-4-H consisted of 91% mannose (Man) and 9% glucuronic acid (GlcA). FT-IR, NMR and methylation analyses indicated that AA-4-H is a branched glucuronomannan. Its main chains are composed of 1, 3-linked alpha-Manp, side chains are single alpha-Manp or alpha-GlcA residues attached to the O-2 and O-6 of Man residues of the main chains. Bioassay indicated that AA-4-H remarkably enhanced B lymphocyte proliferation and increased the production of nitric oxide of macrophages in vitro. Thus, glucuronomannan AA-4-H could be explored as a potential immunostimulation agent.
关键词	Glucuronomannan Auricularia Auricala b Lymphocyte Proliferations Nitric Oxide Macrophages
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	MANNOSE RECEPTOR ; INNATE IMMUNITY ; JUDAE ; POLYSACCHARIDES ; MACROPHAGES ; LIGANDS ; LECTIN ; CELLS
收录类别	SCI
语种	英语
WOS研究方向	Pharmacology & Pharmacy ; Food Science & Technology
WOS类目	Chemistry, Medicinal ; Food Science & Technology
WOS记录号	WOS:000311663700024
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/3590
专题	中国科学院西北高原生物研究所
作者单位	1.NE Normal Univ, Sch Life Sci, Changchun 130024, Peoples R China 2.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 130024, Peoples R China
推荐引用方式 GB/T 7714	Li, Zonghong,Jiang, Dan,Bi, Hongtao,et al. Preparation of a Novel Glucuronomannan from Auricularia auricala and its Immunological Activity[J]. NATURAL PRODUCT COMMUNICATIONS,2012,7(11):1507-1510.
APA	Li, Zonghong,Jiang, Dan,Bi, Hongtao,Liu, Dazheng,Jang, Sungju,&Zhou, Yifa.(2012).Preparation of a Novel Glucuronomannan from Auricularia auricala and its Immunological Activity.NATURAL PRODUCT COMMUNICATIONS,7(11),1507-1510.
MLA	Li, Zonghong,et al."Preparation of a Novel Glucuronomannan from Auricularia auricala and its Immunological Activity".NATURAL PRODUCT COMMUNICATIONS 7.11(2012):1507-1510.