Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS | |
| Ning, Shujing1,2; You, Jinmao1,3; Sun, Zhiwei1; Zhang, Shijuan3; Ji, Zhongyin3 | |
| 2012-10-01 | |
| 发表期刊 | CHROMATOGRAPHIA
 |
| ISSN | 0009-5893 |
| 卷号 | 75期号:19-20页码:1107-1116 |
| 文章类型 | Article |
| 摘要 | A simple and sensitive method for the determination of free aliphatic amines using 10-phenyl-acridone-2-sulfonyl chloride (PASC) as a labeling reagent by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (HPLC-FLD-MS) has been developed. Derivatization conditions including reagent concentration, buffer pH, reaction time and temperature were optimized. PASC reacted with aliphatic amines at 50 A degrees C for 4 min in aqueous acetonitrile (ACN) in the presence of sodiumtetraborate-NaOH buffer (0.10 mol L-1, pH 9.0) to give high yields of PASC-amine derivatives. Derivatives exhibited intense fluorescence with an excitation maximum at lambda(ex) 265 nm and an emission maximum at lambda(em) 418 nm. The separation of derivatives was performed by a reversed-phase Hypersil BDS C8 column in combination with a gradient elution. The identification of derivatives was carried out by online post-column mass spectrometry with atmospheric pressure chemical ionization (APCI) source in positive-ion detection mode. Excellent linear responses were observed with the correlation coefficients of larger than 0.9997, and detection limits (at a signal-to-noise of 3:1) were from 3.0 to 24.3 fmol. Comparing with 10-ethyl-acridine-2-sulfonyl chloride (EASC), PASC exhibited more intense fluorescence and ultraviolet absorbance. The proposed method is sensitive and reproducible for the determination of aliphatic amines from water and soil samples.; A simple and sensitive method for the determination of free aliphatic amines using 10-phenyl-acridone-2-sulfonyl chloride (PASC) as a labeling reagent by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (HPLC-FLD-MS) has been developed. Derivatization conditions including reagent concentration, buffer pH, reaction time and temperature were optimized. PASC reacted with aliphatic amines at 50 A degrees C for 4 min in aqueous acetonitrile (ACN) in the presence of sodiumtetraborate-NaOH buffer (0.10 mol L-1, pH 9.0) to give high yields of PASC-amine derivatives. Derivatives exhibited intense fluorescence with an excitation maximum at lambda(ex) 265 nm and an emission maximum at lambda(em) 418 nm. The separation of derivatives was performed by a reversed-phase Hypersil BDS C8 column in combination with a gradient elution. The identification of derivatives was carried out by online post-column mass spectrometry with atmospheric pressure chemical ionization (APCI) source in positive-ion detection mode. Excellent linear responses were observed with the correlation coefficients of larger than 0.9997, and detection limits (at a signal-to-noise of 3:1) were from 3.0 to 24.3 fmol. Comparing with 10-ethyl-acridine-2-sulfonyl chloride (EASC), PASC exhibited more intense fluorescence and ultraviolet absorbance. The proposed method is sensitive and reproducible for the determination of aliphatic amines from water and soil samples. |
| 关键词 | Hplc/ms Aliphatic Amines 10-phenyl-acridone-2-sulfonyl Chloride Pre-column Derivatization |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; PRESSURE CHEMICAL-IONIZATION ; VOLATILE N-NITROSAMINES ; BIOGENIC-AMINES ; GAS-CHROMATOGRAPHY ; PRECOLUMN DERIVATIZATION ; ENZYMATIC METHOD ; POLYAMINES ; CHLOROFORMATE ; PRODUCTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000309223800003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3606 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273156, Peoples R China 2.Expt High Sch Linqu, Linqu 262600, Peoples R China 3.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ning, Shujing,You, Jinmao,Sun, Zhiwei,et al. Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS[J]. CHROMATOGRAPHIA,2012,75(19-20):1107-1116. |
| APA | Ning, Shujing,You, Jinmao,Sun, Zhiwei,Zhang, Shijuan,&Ji, Zhongyin.(2012).Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS.CHROMATOGRAPHIA,75(19-20),1107-1116. |
| MLA | Ning, Shujing,et al."Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS".CHROMATOGRAPHIA 75.19-20(2012):1107-1116. |
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