Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye

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	Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye
	Wang, Yanqian 1,2,3; Zhang, Bo 2; Liu, Baolong 2; Zhang, Huaigang 2; Liu, Dengcai 1,2
	2012-09-01
发表期刊	JOURNAL OF CEREAL SCIENCE
ISSN	0733-5210
卷号	56 期号:2 页码:282-288
文章类型	Article
摘要	The 75K gamma-secalins encoded by genes present at the locus Sec2 on chromosome 2R are unique to rye and contribute about half of all rye storage proteins. However, there is a lack of sequence information for paralogous genes in this locus. For this study, 59 gamma-secalin paralogous sequences in the Sec2 locus were characterized from a cultivated rye and derived lines after crossing with bread wheat. They had similar structures with conserved sequences in their repetitive regions for the signal peptide, N-terminal, C-terminal and the repeat motif. Their high homology indicated that they originated from an ancestor sequence that existed before the speciation of the genus Secale. Duplication and divergence might have led to the formation of the paralogous genes at Sec2. Besides point mutations, these paralogs showed variations in DNA length due to insertion or deletion events in their repetitive regions. They encoded secalins with deduced molecular weight ranges between 22.2 and 54.5 kDa. These insertion or deletions may be caused by illegitimate recombination and this locus seemed to contribute to increased levels of protein content. However, the incorporation of locus Sec2 may have a negative effect on flour processing quality since it reduced the SDS-sedimentation value. (C) 2012 Elsevier Ltd. All rights reserved.; The 75K gamma-secalins encoded by genes present at the locus Sec2 on chromosome 2R are unique to rye and contribute about half of all rye storage proteins. However, there is a lack of sequence information for paralogous genes in this locus. For this study, 59 gamma-secalin paralogous sequences in the Sec2 locus were characterized from a cultivated rye and derived lines after crossing with bread wheat. They had similar structures with conserved sequences in their repetitive regions for the signal peptide, N-terminal, C-terminal and the repeat motif. Their high homology indicated that they originated from an ancestor sequence that existed before the speciation of the genus Secale. Duplication and divergence might have led to the formation of the paralogous genes at Sec2. Besides point mutations, these paralogs showed variations in DNA length due to insertion or deletion events in their repetitive regions. They encoded secalins with deduced molecular weight ranges between 22.2 and 54.5 kDa. These insertion or deletions may be caused by illegitimate recombination and this locus seemed to contribute to increased levels of protein content. However, the incorporation of locus Sec2 may have a negative effect on flour processing quality since it reduced the SDS-sedimentation value. (C) 2012 Elsevier Ltd. All rights reserved.
关键词	Rye Sec2 Secalin Seed Storage Protein
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	PROLAMIN STORAGE PROTEINS ; SECALE-CEREALE L ; WHEAT 1BL/1RS TRANSLOCATION ; CHROMOSOMAL LOCATIONS ; GAMMA-SECALINS ; MONTANUM GUSS ; GLUTENIN ; SUBUNITS ; RESTRICTION ; MECHANISM
收录类别	SCI
语种	英语
WOS研究方向	Food Science & Technology
WOS类目	Food Science & Technology
WOS记录号	WOS:000309569200023
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/3610
专题	中国科学院西北高原生物研究所
作者单位	1.Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China 2.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Inst Plateau Biol, Xining 810001, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yanqian,Zhang, Bo,Liu, Baolong,et al. Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye[J]. JOURNAL OF CEREAL SCIENCE,2012,56(2):282-288.
APA	Wang, Yanqian,Zhang, Bo,Liu, Baolong,Zhang, Huaigang,&Liu, Dengcai.(2012).Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye.JOURNAL OF CEREAL SCIENCE,56(2),282-288.
MLA	Wang, Yanqian,et al."Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye".JOURNAL OF CEREAL SCIENCE 56.2(2012):282-288.

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