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Analysis of free fatty acids in Notopterygium forbesii Boiss by a novel HPLC method with fluorescence detection
Zhang, Shijuan1,3; You, Jinmao1,2; Zhou, Guoying1; Li, Chunli1,3; Suo, Yourui1
2012-08-30
发表期刊TALANTA
ISSN0039-9140
卷号98页码:95-100
文章类型Article
摘要A new labeling reagent for fatty acids, 1-(9H-carbazol-9-yl) propan-2-yl-methanesulfonate (CPMS), has been synthesized and successfully applied to the HPLC determination of fatty acids in traditional Chinese herb Notopterygium forbesii Boiss. The reaction of CPMS with fatty acids could proceed easily and quickly in the presence of K2CO3 catalyst within 30 min. The derivatives exhibit excellent fluorescence property with excitation and emission wavelengths of 293 nm and 360 nm respectively. The 34 derivatives of fatty acids were separated on a BDS C8 reversed-phase column with gradient elution. Good linear correlations were observed for all fatty acids with correlation coefficients of > 0.996. The detection limits at a signal-to-noise ratio of 3 were in the range 0.032-0.312 mu g g(-1). Free fatty acids in the roots, stem, leaves and petioles of N. forbesii Boiss from different places were analyzed by the developed method. This is the first time that the fatty acids composition of N. forbesii Boiss has been reported. This method also shows powerful potential for the trace analysis of fatty acids or other carboxylic acids from complex samples. (C) 2012 Elsevier B.V. All rights reserved.; A new labeling reagent for fatty acids, 1-(9H-carbazol-9-yl) propan-2-yl-methanesulfonate (CPMS), has been synthesized and successfully applied to the HPLC determination of fatty acids in traditional Chinese herb Notopterygium forbesii Boiss. The reaction of CPMS with fatty acids could proceed easily and quickly in the presence of K2CO3 catalyst within 30 min. The derivatives exhibit excellent fluorescence property with excitation and emission wavelengths of 293 nm and 360 nm respectively. The 34 derivatives of fatty acids were separated on a BDS C8 reversed-phase column with gradient elution. Good linear correlations were observed for all fatty acids with correlation coefficients of > 0.996. The detection limits at a signal-to-noise ratio of 3 were in the range 0.032-0.312 mu g g(-1). Free fatty acids in the roots, stem, leaves and petioles of N. forbesii Boiss from different places were analyzed by the developed method. This is the first time that the fatty acids composition of N. forbesii Boiss has been reported. This method also shows powerful potential for the trace analysis of fatty acids or other carboxylic acids from complex samples. (C) 2012 Elsevier B.V. All rights reserved.
关键词Notopterygium Forbesii Boiss Fatty Acids Fluorescence Hplc
WOS标题词Science & Technology ; Physical Sciences
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; GAS-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; RISK-FACTORS ; FISH-OIL ; EXTRACTION ; OMEGA-3-FATTY-ACIDS ; QUANTIFICATION ; IDENTIFICATION ; QUANTITATION
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000309327600014
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/3618
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Plateau Inst Biol, Xining 810001, Peoples R China
2.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Qufu, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Shijuan,You, Jinmao,Zhou, Guoying,et al. Analysis of free fatty acids in Notopterygium forbesii Boiss by a novel HPLC method with fluorescence detection[J]. TALANTA,2012,98:95-100.
APA Zhang, Shijuan,You, Jinmao,Zhou, Guoying,Li, Chunli,&Suo, Yourui.(2012).Analysis of free fatty acids in Notopterygium forbesii Boiss by a novel HPLC method with fluorescence detection.TALANTA,98,95-100.
MLA Zhang, Shijuan,et al."Analysis of free fatty acids in Notopterygium forbesii Boiss by a novel HPLC method with fluorescence detection".TALANTA 98(2012):95-100.
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