A sensitive and efficient method to systematically detect two biophenols in medicinal herb, herbal products and rat plasma based on thorough study of derivatization and its convenient application to pharmacokinetics with semi-automated device

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	A sensitive and efficient method to systematically detect two biophenols in medicinal herb, herbal products and rat plasma based on thorough study of derivatization and its convenient application to pharmacokinetics with semi-automated device
	Chen, Guang 1,2,3,4; Li, Jun 3,4; Zhang, Shijuan 1,2; Song, Cuihua 3,4; Li, Guoliang 1,2; Sun, Zhiwei 3,4; Suo, Yourui 1; You, Jinmao 1,3,4
	2012-08-03
发表期刊	JOURNAL OF CHROMATOGRAPHY A
ISSN	0021-9673
卷号	1249 页码:190-200
文章类型	Article
摘要	A sensitive and efficient method using a semi-automated pretreatment device, pre-column derivatization, multivariate optimization and high performance liquid chromatography with fluorescence and mass spectrometric detection was developed and validated for the systematic determination of two biophenols in four herb-related samples (medicinal herb; herbal products in tablet, capsule and oral liquid forms) and plasma samples after oral administration to rat. Only micro-sampling of 20 mu L blood was needed for the analysis, and the pretreatment procedure including blood collection, derivatization by 10-ethyl-acridine-3-sulfonyl chloride (EASC) and injection to the sampling vials was efficiently finished in 10 min with no cumbersome and complicated operation. The novel application of artificial neural network (ANN) coupled with genetic algorithm (GA) to optimization of derivatization condition was executed and compared with the classical response surface methodology (RSM). The optimal condition for derivatization was validated by multi-criteria and nonparametric tests and used successfully to achieve the higher sensitivity (limit of detection: 0.6 and 0.8 ng/mL). The limit of reactant concentration (LORC) was put forward for derivatization method for the first time, and the lower values (2.0-2.7 ng/mL) provided the guarantee for the trace detection with the micro samples (<50 mu L) required. The results of validation including selectivity, sensitivity, linearity, accuracy, precision, recovery, matrix effect and stability demonstrated the advantages of this method. The pharmacokinetic study of major bioactive components salidroside and p-tyrosol in herb Rhodiola crenulata and its products was more conveniently performed in 25 min. The established method could be the sensitive and efficient alternative method for the systematic detection of bioactive components in series of drug carriers from raw herb to herbal products and to blood in medical research. And the approaches of the thorough study played the guiding role in seeking a novel analytical method. (C) 2012 Elsevier B.V. All rights reserved.; A sensitive and efficient method using a semi-automated pretreatment device, pre-column derivatization, multivariate optimization and high performance liquid chromatography with fluorescence and mass spectrometric detection was developed and validated for the systematic determination of two biophenols in four herb-related samples (medicinal herb; herbal products in tablet, capsule and oral liquid forms) and plasma samples after oral administration to rat. Only micro-sampling of 20 mu L blood was needed for the analysis, and the pretreatment procedure including blood collection, derivatization by 10-ethyl-acridine-3-sulfonyl chloride (EASC) and injection to the sampling vials was efficiently finished in 10 min with no cumbersome and complicated operation. The novel application of artificial neural network (ANN) coupled with genetic algorithm (GA) to optimization of derivatization condition was executed and compared with the classical response surface methodology (RSM). The optimal condition for derivatization was validated by multi-criteria and nonparametric tests and used successfully to achieve the higher sensitivity (limit of detection: 0.6 and 0.8 ng/mL). The limit of reactant concentration (LORC) was put forward for derivatization method for the first time, and the lower values (2.0-2.7 ng/mL) provided the guarantee for the trace detection with the micro samples (<50 mu L) required. The results of validation including selectivity, sensitivity, linearity, accuracy, precision, recovery, matrix effect and stability demonstrated the advantages of this method. The pharmacokinetic study of major bioactive components salidroside and p-tyrosol in herb Rhodiola crenulata and its products was more conveniently performed in 25 min. The established method could be the sensitive and efficient alternative method for the systematic detection of bioactive components in series of drug carriers from raw herb to herbal products and to blood in medical research. And the approaches of the thorough study played the guiding role in seeking a novel analytical method. (C) 2012 Elsevier B.V. All rights reserved.
关键词	Biophenol Medicinal Herb Derivatization Multivariate Optimization Artificial Neural Network Pharmacokinetics
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; ARTIFICIAL NEURAL-NETWORKS ; QUANTITATIVE-DETERMINATION ; RHODIOLA-CRENULATA ; GENETIC ALGORITHMS ; MASS-SPECTROMETRY ; SALIDROSIDE ; FLUORESCENCE ; TYROSOL ; HPLC
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000306818700020
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/3626
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 4.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Chen, Guang,Li, Jun,Zhang, Shijuan,et al. A sensitive and efficient method to systematically detect two biophenols in medicinal herb, herbal products and rat plasma based on thorough study of derivatization and its convenient application to pharmacokinetics with semi-automated device[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1249:190-200.
APA	Chen, Guang.,Li, Jun.,Zhang, Shijuan.,Song, Cuihua.,Li, Guoliang.,...&You, Jinmao.(2012).A sensitive and efficient method to systematically detect two biophenols in medicinal herb, herbal products and rat plasma based on thorough study of derivatization and its convenient application to pharmacokinetics with semi-automated device.JOURNAL OF CHROMATOGRAPHY A,1249,190-200.
MLA	Chen, Guang,et al."A sensitive and efficient method to systematically detect two biophenols in medicinal herb, herbal products and rat plasma based on thorough study of derivatization and its convenient application to pharmacokinetics with semi-automated device".JOURNAL OF CHROMATOGRAPHY A 1249(2012):190-200.

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