Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography | |
| Jia, Jing1,2; Chen, Tao1,2; Wang, Ping1,2; Chen, Guichen1; You, Jinmao1; Liu, Yongjun1; Li, Yulin1 | |
| 2012-07-01 | |
| 发表期刊 | PHYTOCHEMICAL ANALYSIS
 |
| ISSN | 0958-0344 |
| 卷号 | 23期号:4页码:332-336 |
| 文章类型 | Article |
| 摘要 | Introduction Xanthones, the primary constituents of Swertia mussotii, are known to possess a variety of biological activities, including anti-depressant, anti-leukaemic, anti-tumour, anti-tubercular, choleretic, diuretic, anti-microbial, anti-fungal, anti-inflammatory, anti-viral, cardiotonic and hypoglycemic properties. However, high performance, environmentally friendly methods for isolating and purifying xanthones from S. mussotii are not currently available. Objective To develop a high performance and environmentally friendly method for the preparative separation of xanthones methylswertianin, swerchirin and decussatin from S. mussotii using high-speed counter-current chromatography (HSCCC). Methodology A solvent system composed of n-hexane:ethyl acatate:methanol:water (5:5:10:4, v/v/v/v) was developed for the separation method. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5?mL/min, a rotation speed of 800?rpm and a temperature of 25 degrees C. Results Using the described method, 8?mg of methylswertianin, 21?mg of swerchirin and 11?mg of decussatin with purities of over 98% could be isolated from a 150?mg crude sample. They were identified by 1H-NMR and 13?C-NMR analysis. Conclusion Three xanthones in Swertia mussotii could be systematically isolated and purified using HSCCC. Copyright (C) 2011 John Wiley & Sons, Ltd.; Introduction Xanthones, the primary constituents of Swertia mussotii, are known to possess a variety of biological activities, including anti-depressant, anti-leukaemic, anti-tumour, anti-tubercular, choleretic, diuretic, anti-microbial, anti-fungal, anti-inflammatory, anti-viral, cardiotonic and hypoglycemic properties. However, high performance, environmentally friendly methods for isolating and purifying xanthones from S. mussotii are not currently available. Objective To develop a high performance and environmentally friendly method for the preparative separation of xanthones methylswertianin, swerchirin and decussatin from S. mussotii using high-speed counter-current chromatography (HSCCC). Methodology A solvent system composed of n-hexane:ethyl acatate:methanol:water (5:5:10:4, v/v/v/v) was developed for the separation method. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5?mL/min, a rotation speed of 800?rpm and a temperature of 25 degrees C. Results Using the described method, 8?mg of methylswertianin, 21?mg of swerchirin and 11?mg of decussatin with purities of over 98% could be isolated from a 150?mg crude sample. They were identified by 1H-NMR and 13?C-NMR analysis. Conclusion Three xanthones in Swertia mussotii could be systematically isolated and purified using HSCCC. Copyright (C) 2011 John Wiley & Sons, Ltd. |
| 关键词 | High-speed Counter-current Chromatography Xanthone Methylswertianin Swerchirin Decussatin Swertia Mussotii |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 关键词[WOS] | PURIFICATION ; XANTHONES ; ISOPSORALEN ; PSORALEN ; RADIX |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Plant Sciences ; Chemistry, Analytical |
| WOS记录号 | WOS:000305571500007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3652 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Jing,Chen, Tao,Wang, Ping,et al. Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography[J]. PHYTOCHEMICAL ANALYSIS,2012,23(4):332-336. |
| APA | Jia, Jing.,Chen, Tao.,Wang, Ping.,Chen, Guichen.,You, Jinmao.,...&Li, Yulin.(2012).Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography.PHYTOCHEMICAL ANALYSIS,23(4),332-336. |
| MLA | Jia, Jing,et al."Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography".PHYTOCHEMICAL ANALYSIS 23.4(2012):332-336. |
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