Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Novel HMW glutenin genes from Aegilops tauschii and their unique structures | |
Chen, Wen-Jie1; Yuan, Zhong-Wei1; Zhang, Lian-Quan1; Fan, Xing1; Yan, Ze-Hong1; Wang, Ji-Rui1; Zheng, You-Liang1; Zhang, Huai-Gang2; Liu, Deng-Cai1,2 | |
2012-06-01 | |
发表期刊 | GENES & GENOMICS |
ISSN | 1976-9571 |
卷号 | 34期号:3页码:339-343 |
文章类型 | Article |
摘要 | A pair of novel high-molecular-weight glutenin subunits (HMW-GS) 1Dx5.3(t) and 1Dy12.1**(t) were revealed and characterized from Ae. tauschii accession PI554324. SDS-PAGE band of 1Dx5.3(t) was between those of 1Bx6 and 1Bx7, while 1Dy12.1**(t) with slightly faster migration rate than that of 1Dy12. The lengths of 1Dx5.3 (t) and 1Dy12.1** (t) were 2115 bp and 1986 bp, encoding 703 and 660 amino acid residues, respectively. Their authenticity was confirmed by successful expression of the coding regions in Escherichia coli. 1Dx5.3(t) is the shortest of the known Dx-type alleles. 1Dy12.1**(t) is also a special subunit since it has an additional cysteine in the front of the central repetitive domain. This cysteine that is not existed in previously reported Dy-type genes may be useful for improving bread wheat quality. Median-joining Network analysis indicated that 1Dy12.1** (t) may be a key site in the genealogy of the Glu-Dy.; A pair of novel high-molecular-weight glutenin subunits (HMW-GS) 1Dx5.3(t) and 1Dy12.1**(t) were revealed and characterized from Ae. tauschii accession PI554324. SDS-PAGE band of 1Dx5.3(t) was between those of 1Bx6 and 1Bx7, while 1Dy12.1**(t) with slightly faster migration rate than that of 1Dy12. The lengths of 1Dx5.3 (t) and 1Dy12.1** (t) were 2115 bp and 1986 bp, encoding 703 and 660 amino acid residues, respectively. Their authenticity was confirmed by successful expression of the coding regions in Escherichia coli. 1Dx5.3(t) is the shortest of the known Dx-type alleles. 1Dy12.1**(t) is also a special subunit since it has an additional cysteine in the front of the central repetitive domain. This cysteine that is not existed in previously reported Dy-type genes may be useful for improving bread wheat quality. Median-joining Network analysis indicated that 1Dy12.1** (t) may be a key site in the genealogy of the Glu-Dy. |
关键词 | Aegilops Tauschii Bacterial Expression Hmw-gs Phylogenetic Analysis |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
关键词[WOS] | MOLECULAR-WEIGHT SUBUNITS ; BREAD-MAKING QUALITY ; D-GENOME ; TRITICUM-TAUSCHII ; ALLELIC VARIATION ; HEXAPLOID WHEAT ; IDENTIFICATION ; EXPRESSION ; PROTEINS |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity |
WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity |
WOS记录号 | WOS:000305695300012 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/3660 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Sichuan, Peoples R China 2.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Wen-Jie,Yuan, Zhong-Wei,Zhang, Lian-Quan,et al. Novel HMW glutenin genes from Aegilops tauschii and their unique structures[J]. GENES & GENOMICS,2012,34(3):339-343. |
APA | Chen, Wen-Jie.,Yuan, Zhong-Wei.,Zhang, Lian-Quan.,Fan, Xing.,Yan, Ze-Hong.,...&Liu, Deng-Cai.(2012).Novel HMW glutenin genes from Aegilops tauschii and their unique structures.GENES & GENOMICS,34(3),339-343. |
MLA | Chen, Wen-Jie,et al."Novel HMW glutenin genes from Aegilops tauschii and their unique structures".GENES & GENOMICS 34.3(2012):339-343. |
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