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Novel HMW glutenin genes from Aegilops tauschii and their unique structures
Chen, Wen-Jie1; Yuan, Zhong-Wei1; Zhang, Lian-Quan1; Fan, Xing1; Yan, Ze-Hong1; Wang, Ji-Rui1; Zheng, You-Liang1; Zhang, Huai-Gang2; Liu, Deng-Cai1,2
2012-06-01
发表期刊GENES & GENOMICS
ISSN1976-9571
卷号34期号:3页码:339-343
文章类型Article
摘要A pair of novel high-molecular-weight glutenin subunits (HMW-GS) 1Dx5.3(t) and 1Dy12.1**(t) were revealed and characterized from Ae. tauschii accession PI554324. SDS-PAGE band of 1Dx5.3(t) was between those of 1Bx6 and 1Bx7, while 1Dy12.1**(t) with slightly faster migration rate than that of 1Dy12. The lengths of 1Dx5.3 (t) and 1Dy12.1** (t) were 2115 bp and 1986 bp, encoding 703 and 660 amino acid residues, respectively. Their authenticity was confirmed by successful expression of the coding regions in Escherichia coli. 1Dx5.3(t) is the shortest of the known Dx-type alleles. 1Dy12.1**(t) is also a special subunit since it has an additional cysteine in the front of the central repetitive domain. This cysteine that is not existed in previously reported Dy-type genes may be useful for improving bread wheat quality. Median-joining Network analysis indicated that 1Dy12.1** (t) may be a key site in the genealogy of the Glu-Dy.; A pair of novel high-molecular-weight glutenin subunits (HMW-GS) 1Dx5.3(t) and 1Dy12.1**(t) were revealed and characterized from Ae. tauschii accession PI554324. SDS-PAGE band of 1Dx5.3(t) was between those of 1Bx6 and 1Bx7, while 1Dy12.1**(t) with slightly faster migration rate than that of 1Dy12. The lengths of 1Dx5.3 (t) and 1Dy12.1** (t) were 2115 bp and 1986 bp, encoding 703 and 660 amino acid residues, respectively. Their authenticity was confirmed by successful expression of the coding regions in Escherichia coli. 1Dx5.3(t) is the shortest of the known Dx-type alleles. 1Dy12.1**(t) is also a special subunit since it has an additional cysteine in the front of the central repetitive domain. This cysteine that is not existed in previously reported Dy-type genes may be useful for improving bread wheat quality. Median-joining Network analysis indicated that 1Dy12.1** (t) may be a key site in the genealogy of the Glu-Dy.
关键词Aegilops Tauschii Bacterial Expression Hmw-gs Phylogenetic Analysis
WOS标题词Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]MOLECULAR-WEIGHT SUBUNITS ; BREAD-MAKING QUALITY ; D-GENOME ; TRITICUM-TAUSCHII ; ALLELIC VARIATION ; HEXAPLOID WHEAT ; IDENTIFICATION ; EXPRESSION ; PROTEINS
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号WOS:000305695300012
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/3660
专题中国科学院西北高原生物研究所
作者单位1.Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Sichuan, Peoples R China
2.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China
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GB/T 7714
Chen, Wen-Jie,Yuan, Zhong-Wei,Zhang, Lian-Quan,et al. Novel HMW glutenin genes from Aegilops tauschii and their unique structures[J]. GENES & GENOMICS,2012,34(3):339-343.
APA Chen, Wen-Jie.,Yuan, Zhong-Wei.,Zhang, Lian-Quan.,Fan, Xing.,Yan, Ze-Hong.,...&Liu, Deng-Cai.(2012).Novel HMW glutenin genes from Aegilops tauschii and their unique structures.GENES & GENOMICS,34(3),339-343.
MLA Chen, Wen-Jie,et al."Novel HMW glutenin genes from Aegilops tauschii and their unique structures".GENES & GENOMICS 34.3(2012):339-343.
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