Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS | |
| Xie, Yinfeng1; Li, Guoliang2,3; You, Jinmao1,2; Bai, Xinwei1; Wang, Chengyan1; Zhang, Lin1; Zhao, Fuhua1; Wu, Xiaoyun1; Ji, Zhongyin1; Sun, Zhiwei2,3 | |
| 2012-06-01 | |
| 发表期刊 | CHROMATOGRAPHIA
 |
| ISSN | 0009-5893 |
| 卷号 | 75期号:11-12页码:571-583 |
| 文章类型 | Article |
| 摘要 | A new fluorescence labeling reagent 2-(12-benzo[b]acridin-5(12H)-yl)-acetohydrazide (BAAH) has been designed for fatty acids labeling. Eleven fatty acids containing seven saturated and four unsaturated fatty acids were used to evaluate the analytical potential of this reagent. The labeling reaction of BAAH with fatty acids was completed at 85 A degrees C for 60 min using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC center dot HCl) as the condensing agent. Separation of the derivatized fatty acids was carried out on a reversed-phase Thermo Hypersil Gold C18 column (4.6 mm x 250 mm, 5 mu m) in combination with a gradient elution with a good baseline resolution. The fluorescence excitation and emission wavelengths were set at lambda(ex) 280 and lambda(em) 510 nm, respectively. The identification was carried out by the online APCI-MS in positive-ion detection mode. Linear correlation coefficients for all fatty acid derivatives were of > 0.9994. Detection limits, at a signal-to-noise ratio of 3:1, were 3.89-12.5 nmol L-1 for the labeled fatty acids. The developed method was successfully applied to the accurate determination of fatty acids in five traditional Chinese herbs with satisfactory results.; A new fluorescence labeling reagent 2-(12-benzo[b]acridin-5(12H)-yl)-acetohydrazide (BAAH) has been designed for fatty acids labeling. Eleven fatty acids containing seven saturated and four unsaturated fatty acids were used to evaluate the analytical potential of this reagent. The labeling reaction of BAAH with fatty acids was completed at 85 A degrees C for 60 min using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC center dot HCl) as the condensing agent. Separation of the derivatized fatty acids was carried out on a reversed-phase Thermo Hypersil Gold C18 column (4.6 mm x 250 mm, 5 mu m) in combination with a gradient elution with a good baseline resolution. The fluorescence excitation and emission wavelengths were set at lambda(ex) 280 and lambda(em) 510 nm, respectively. The identification was carried out by the online APCI-MS in positive-ion detection mode. Linear correlation coefficients for all fatty acid derivatives were of > 0.9994. Detection limits, at a signal-to-noise ratio of 3:1, were 3.89-12.5 nmol L-1 for the labeled fatty acids. The developed method was successfully applied to the accurate determination of fatty acids in five traditional Chinese herbs with satisfactory results. |
| 关键词 | Hplc Pre-column Derivatization 2-(12-benzo[b]Acridin-5(12h)-yl)-acetohydrazide (Baah) Fatty Acids Traditional Chinese Herbs |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; CYATHULA-OFFICINALIS KUAN ; INDUCED FLUORESCENCE DETECTION ; Y79 RETINOBLASTOMA CELLS ; CARBOXYLIC-ACIDS ; RHODIOLA-CRENULATA ; APOCYNUM-VENETUM ; CAPILLARY-ELECTROPHORESIS ; DERIVATIZATION REAGENT ; RAT PLASMA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000304557300002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/3662 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Qinghai, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Yinfeng,Li, Guoliang,You, Jinmao,et al. A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS[J]. CHROMATOGRAPHIA,2012,75(11-12):571-583. |
| APA | Xie, Yinfeng.,Li, Guoliang.,You, Jinmao.,Bai, Xinwei.,Wang, Chengyan.,...&Sun, Zhiwei.(2012).A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS.CHROMATOGRAPHIA,75(11-12),571-583. |
| MLA | Xie, Yinfeng,et al."A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS".CHROMATOGRAPHIA 75.11-12(2012):571-583. |
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